Aquatic macroinvertebrates are regularly used for assessing the health of Australian river systems. These assessments rely on accurate identifications of the macroinvertebrate fauna present within monitoring sites. DNA barcoding is a method of obtaining genetic data from specimens that can be used to accurately identify specimens to species. However, in order to use DNA barcoding as a routine species identification tool, it is important to develop a database of identified specimens and their DNA barcodes.