File(s) stored somewhere else
Please note: Linked content is NOT stored on La Trobe and we can't guarantee its availability, quality, security or accept any liability.
Vitamin c and e treatment blunts sprint interval training–induced changes in inflammatory mediator-, calcium-, and mitochondria-related signaling in recreationally active elderly humans
journal contributionposted on 2020-11-23, 01:15 authored by Victoria Wyckelsma, T Venckunas, M Brazaitis, S Gastaldello, A Snieckus, N Eimantas, N Baranauskiene, A Subocius, A Skurvydas, M Pääsuke, H Gapeyeva, P Kaasik, R Pääsuke, J Jürimäe, BA Graf, B Kayser, N Place, DC Andersson, S Kamandulis, H Westerblad
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants vitamin C and E. Recreationally active elderly (mean age 65) men received either vitamin C (1 g/day) and vitamin E (235 mg/day) or placebo. Training consisted of nine SIT sessions (three sessions/week for three weeks of 4–6 repetitions of 30-s all-out cycling sprints) interposed by 4 min rest. Vastus lateralis muscle biopsies were taken before, 1 h after, and 24 h after the first and last SIT sessions. At the end of the three weeks of training, SIT-induced changes in relative mRNA expression of reactive oxygen/nitrogen species (ROS)and mitochondria-related proteins, inflammatory mediators, and the sarcoplasmic reticulum Ca2+ channel, the ryanodine receptor 1 (RyR1), were blunted in the vitamin treated group. Western blots frequently showed a major (>50%) decrease in the full-length expression of RyR1 24 h after SIT sessions; in the trained state, vitamin treatment seemed to provide protection against this severe RyR1 modification. Power at exhaustion during an incremental cycling test was increased by ~5% at the end of the training period, whereas maximal oxygen uptake remained unchanged; vitamin treatment did not affect these measures. In conclusion, treatment with the antioxidants vitamin C and E blunts SIT-induced cellular signaling in skeletal muscle of elderly individuals, while the present training regimen was too short or too intense for the changes in signaling to be translated into a clear-cut change in physical performance.
This work was supported by grants from the Research Council of Lithuania (SEN-08/2016), the Estonian Ministry of Education and Science (IUT20-58), the Swedish Research Council (K2014-52X-10842-21-5), and the Swedish Research Council for Sport Science (FO2016-033).
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
Rights StatementThe Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.
CategoriesNo categories selected
Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyChemistry, MedicinalFood Science & TechnologyPharmacology & Pharmacysprint interval traininghigh-intensity interval trainingagingendurance exerciseskeletal muscleantioxidant treatmentreactive oxygen/nitrogen speciescalciuminflammatory mediatorsSKELETAL-MUSCLEPHYSICAL-EXERCISERISK-FACTORSLOW-VOLUMEINCREASESBIOGENESISMECHANISMSFATIGUEADAPTATIONSCAPACITY