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Vitamin c and e treatment blunts sprint interval training–induced changes in inflammatory mediator-, calcium-, and mitochondria-related signaling in recreationally active elderly humans
journal contributionposted on 23.11.2020, 01:15 authored by Victoria Wyckelsma, T Venckunas, M Brazaitis, S Gastaldello, A Snieckus, N Eimantas, N Baranauskiene, A Subocius, A Skurvydas, M Pääsuke, H Gapeyeva, P Kaasik, R Pääsuke, J Jürimäe, BA Graf, B Kayser, N Place, DC Andersson, S Kamandulis, H Westerblad
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Sprint interval training (SIT) has emerged as a time-efficient training regimen for young individuals. Here, we studied whether SIT is effective also in elderly individuals and whether the training response was affected by treatment with the antioxidants vitamin C and E. Recreationally active elderly (mean age 65) men received either vitamin C (1 g/day) and vitamin E (235 mg/day) or placebo. Training consisted of nine SIT sessions (three sessions/week for three weeks of 4–6 repetitions of 30-s all-out cycling sprints) interposed by 4 min rest. Vastus lateralis muscle biopsies were taken before, 1 h after, and 24 h after the first and last SIT sessions. At the end of the three weeks of training, SIT-induced changes in relative mRNA expression of reactive oxygen/nitrogen species (ROS)and mitochondria-related proteins, inflammatory mediators, and the sarcoplasmic reticulum Ca2+ channel, the ryanodine receptor 1 (RyR1), were blunted in the vitamin treated group. Western blots frequently showed a major (>50%) decrease in the full-length expression of RyR1 24 h after SIT sessions; in the trained state, vitamin treatment seemed to provide protection against this severe RyR1 modification. Power at exhaustion during an incremental cycling test was increased by ~5% at the end of the training period, whereas maximal oxygen uptake remained unchanged; vitamin treatment did not affect these measures. In conclusion, treatment with the antioxidants vitamin C and E blunts SIT-induced cellular signaling in skeletal muscle of elderly individuals, while the present training regimen was too short or too intense for the changes in signaling to be translated into a clear-cut change in physical performance.
This work was supported by grants from the Research Council of Lithuania (SEN-08/2016), the Estonian Ministry of Education and Science (IUT20-58), the Swedish Research Council (K2014-52X-10842-21-5), and the Swedish Research Council for Sport Science (FO2016-033).
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
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Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyChemistry, MedicinalFood Science & TechnologyPharmacology & Pharmacysprint interval traininghigh-intensity interval trainingagingendurance exerciseskeletal muscleantioxidant treatmentreactive oxygen/nitrogen speciescalciuminflammatory mediatorsSKELETAL-MUSCLEPHYSICAL-EXERCISERISK-FACTORSLOW-VOLUMEINCREASESBIOGENESISMECHANISMSFATIGUEADAPTATIONSCAPACITY