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Vitamin D Receptor Regulates the Expression of the Grainyhead-Like 1 Gene

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posted on 2024-08-29, 06:43 authored by Agnieszka Taracha-Wisniewska, Emma ParksEmma Parks, Michal Miller, Lidia Lipinska-Zubrycka, Sebastian DworkinSebastian Dworkin, Tomasz Wilanowski
Vitamin D plays an important pleiotropic role in maintaining global homeostasis of the human body. Its functions go far beyond skeletal health, playing a crucial role in a plethora of cellular functions, as well as in extraskeletal health, ensuring the proper functioning of multiple human organs, including the skin. Genes from the Grainyhead-like (GRHL) family code for transcription factors necessary for the development and maintenance of various epithelia. Even though they are involved in many processes regulated by vitamin D, a direct link between vitamin D-mediated cellular pathways and GRHL genes has never been described. We employed various bioinformatic methods, quantitative real-time PCR, chromatin immunoprecipitation, reporter gene assays, and calcitriol treatments to investigate this issue. We report that the vitamin D receptor (VDR) binds to a regulatory region of the Grainyhead-like 1 (GRHL1) gene and regulates its expression. Ectopic expression of VDR and treatment with calcitriol alters the expression of the GRHL1 gene. The evidence presented here indicates a role of VDR in the regulation of expression of GRHL1 and correspondingly a role of GRHL1 in mediating the actions of vitamin D.

Funding

This research was funded by the National Science Centre (Poland), grant 2016/21/B/NZ1/00279.

History

Publication Date

2024-07-19

Journal

International Journal of Molecular Sciences

Volume

25

Issue

14

Article Number

7913

Pagination

16p.

Publisher

Multidisciplinary Digital Publishing Institute

ISSN

1422-0067

Rights Statement

© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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