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Visualisation and measurement of the local absorption coefficients.pdf (1.19 MB)

Visualization and measurement of the local absorption coefficients of single bilayer phospholipid membranes using scanning near-field optical microscopy

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posted on 2021-02-15, 23:50 authored by Arif SiddiqueeArif Siddiquee, Imad Younus Hasan, Shibiao Wei, Daniel Langley, Eugeniu BalaurEugeniu Balaur, Chen Liu, Jiao Lin, Brian AbbeyBrian Abbey, Adam MechlerAdam Mechler, Shanshan KouShanshan Kou

Here we report the results of shear-mode thicknesses and absorption coefficient measurements made on neat membranes using scanning near-field optical microscopy (SNOM). Biomimic neat membranes composed of two different types of phoshpholipid molecules: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were found to exhibit different absorption coefficients under the SNOM. The localization of the lipids could be identified and correlated to the morphology of the membrane domains indicating that SNOM can be an effective and accurate approach for the label-free characterization of the structure-function relationships in cell membranes.

Funding

La Trobe University (Research Infrastructure Grant, RFA Understanding Disease (UD) Partnership Grant).

History

Publication Date

2019-12-01

Journal

Biomedical Optics Express

Volume

10

Issue

12

Pagination

11p. (p. 6569-6569)

Publisher

The Optical Society

ISSN

2156-7085

Rights Statement

© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement. An OSA-formatted open access journal article PDF may be governed by the OSA Open Access Publishing Agreement signed by the author and any applicable copyright laws. Authors and readers may use, reuse, and build upon the article, or use it for text or data mining without asking prior permission from the publisher or the Author(s), as long as the purpose is non-commercial and appropriate attribution is maintained.

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