Visualization and measurement of the local absorption coefficients of single bilayer phospholipid membranes using scanning near-field optical microscopy
journal contributionposted on 2021-02-15, 23:50 authored by Arif SiddiqueeArif Siddiquee, Imad Younus Hasan, Shibiao Wei, Daniel Langley, Eugeniu BalaurEugeniu Balaur, Chen Liu, Jiao Lin, Brian AbbeyBrian Abbey, Adam MechlerAdam Mechler, Shanshan KouShanshan Kou
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement Here we report the results of shear-mode thicknesses and absorption coefficient measurements made on neat membranes using scanning near-field optical microscopy (SNOM). Biomimic neat membranes composed of two different types of phoshpholipid molecules: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were found to exhibit different absorption coefficients under the SNOM. The localization of the lipids could be identified and correlated to the morphology of the membrane domains indicating that SNOM can be an effective and accurate approach for the label-free characterization of the structure-function relationships in cell membranes.