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Visualization and measurement of the local absorption coefficients of single bilayer phospholipid membranes using scanning near-field optical microscopy

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journal contribution
posted on 2021-02-15, 23:50 authored by Arif SiddiqueeArif Siddiquee, Imad Younus Hasan, Shibiao Wei, Daniel Langley, Eugeniu BalaurEugeniu Balaur, Chen Liu, Jiao Lin, Brian AbbeyBrian Abbey, Adam MechlerAdam Mechler, Shanshan KouShanshan Kou
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement Here we report the results of shear-mode thicknesses and absorption coefficient measurements made on neat membranes using scanning near-field optical microscopy (SNOM). Biomimic neat membranes composed of two different types of phoshpholipid molecules: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were found to exhibit different absorption coefficients under the SNOM. The localization of the lipids could be identified and correlated to the morphology of the membrane domains indicating that SNOM can be an effective and accurate approach for the label-free characterization of the structure-function relationships in cell membranes.

Funding

La TrobeUniversity (Research Infrastructure Grant, RFAUnderstanding Disease (UD) Partnership Grant).

History

Publication Date

2019-01-01

Journal

Biomedical Optics Express

Volume

10

Issue

12

Pagination

11p. (p. 6569-6569)

Publisher

The Optical Society

ISSN

2156-7085

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.