La Trobe
1170377_Khantisitthiporn,O_2021.pdf (3.23 MB)
Download file

Viperin interacts with PEX19 to mediate peroxisomal augmentation of the innate antiviral response

Download (3.23 MB)
journal contribution
posted on 07.07.2021, 00:35 by O Khantisitthiporn, B Shue, NS Eyre, CW Nash, L Turnbull, CB Whitchurch, KH Van der Hoek, Karla HelbigKarla Helbig, MR Beard
Peroxisomes are recognized as significant platforms for the activation of antiviral innate immunity where stimulation of the key adapter molecule mitochondrial antiviral signaling protein (MAVS) within the RIG-I like receptor (RLR) pathway culminates in the up-regulation of hundreds of ISGs, some of which drive augmentation of multiple innate sensing pathways. However, whether ISGs can augment peroxisome-driven RLR signaling is currently unknown. Using a proteomics-based screening approach, we identified Pex19 as a binding partner of the ISG viperin. Viperin colocalized with numerous peroxisomal proteins and its interaction with Pex19 was in close association with lipid droplets, another emerging innate signaling platform. Augmentation of the RLR pathway by viperin was lost when Pex19 expression was reduced. Expression of organelle-specific MAVS demonstrated that viperin requires both mitochondria and peroxisome MAVS for optimal induction of IFN-β. These results suggest that viperin is required to enhance the antiviral cellular response with a possible role to position the peroxisome at the mitochondrial/MAM MAVS signaling synapse, furthering our understanding of the importance of multiple organelles driving the innate immune response against viral infection.

History

Publication Date

01/07/2021

Journal

Life Science Alliance

Volume

4

Issue

7

Pagination

(p. e202000915-e202000915)

Publisher

Life Science Alliance LLC

ISSN

2575-1077

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

Usage metrics

Categories

Licence

Exports