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Use of Field Based Loop Mediated Isothermal Amplification (LAMP) Technology for a Prevalence Survey and Proof of Freedom Survey for African Swine Fever in Timor-Leste in 2019

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journal contribution
posted on 03.08.2021, 03:03 by DE Phillips, PT Mee, SE Lynch, F da Conceição, J Bendita da Costa Jong, Grant RawlinGrant Rawlin
African Swine Fever (ASF) has been spreading in numerous southeast Asian countries since a major incursion in mainland China in 2018. Timor-Leste confirmed an outbreak of ASF in September 2019 which resulted in high mortalities in affected pigs. Pigs in Timor-Leste are the second most common type of livestock kept by villagers and represent a traditionally important source of income and prestige for householders. In order to understand the extent of ASF infected villages in Timor-Leste a prevalence survey was designed and conducted in November-December 2019. Timor-Leste has limited laboratory facilities and access to qPCR diagnostic tests. Therefore, a loop mediated isothermal amplification (LAMP) assay was used to detect ASF positive blood samples collected during the prevalence survey. The LAMP assay was proven to be a robust, highly specific and sensitive laboratory test for ASF suitable for use in the field and where there are limited laboratory facilities. The results of the prevalence survey allowed the extent of the ASF incursion to be delineated and the introduction of a disease response strategy to limit the spread of ASF and assist in the recovery of the pig population in Timor-Leste.

Funding

We thank the Department of Agriculture and the Department of Jobs Precincts and Regions for their support by providing funding for this research and travel cost to Timor-Leste. We thank Ian Shurvell from Department of Agriculture and the Department of Jobs Precincts and Regions for assistance with mapping images.

History

Publication Date

21/06/2021

Journal

Frontiers in Veterinary Science

Volume

8

Article Number

ARTN 672048

Pagination

9p.

Publisher

FRONTIERS MEDIA SA

ISSN

2297-1769

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