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Understanding DNA organization, damage, and repair with super-resolution fluorescence microscopy

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journal contribution
posted on 24.06.2021, 01:47 by EL Miriklis, AM Rozario, E Rothenberg, TDM Bell, Donna WhelanDonna Whelan
Super-resolution microscopy (SRM) comprises a suite of techniques well-suited to probing the nanoscale landscape of genomic function and dysfunction. Offering the specificity and sensitivity that has made conventional fluorescence microscopy a cornerstone technique of biological research, SRM allows for spatial resolutions as good as 10 nanometers. Moreover, single molecule localization microscopies (SMLMs) enable examination of individual molecular targets and nanofoci allowing for the characterization of subpopulations within a single cell. This review describes how key advances in both SRM techniques and sample preparation have enabled unprecedented insights into DNA structure and function, and highlights many of these new discoveries. Ongoing development and application of these novel, highly interdisciplinary SRM assays will continue to expand the toolbox available for research into the nanoscale genomic landscape.

Funding

DRW is the recipient of an Australian Research Council Discovery Early Career Research Award (DE200100584) funded by the Australian Government. TDMB acknowledges support from the Australian Research Council (DP170104477). ER acknowledges funding support from the National Institute of Health (1R35GM134947-01, 1P01CA247773-01/5491), American Cancer Society (RSG DMC-16-241-01-DMC) and the V foundation for Cancer Research (D2018-020).

History

Publication Date

01/07/2021

Journal

Methods and Applications in Fluorescence

Volume

9

Issue

3

Article Number

032002

Pagination

21p.

Publisher

IOP Publishing

ISSN

2050-6120

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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