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Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination

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journal contribution
posted on 07.05.2021, 00:07 by SL Whiteley, CE Holleley, S Wagner, J Blackburn, IW Deveson, Jennifer GravesJennifer Graves, A Georges
How temperature determines sex remains unknown. A recent hypothesis proposes that conserved cellular mechanisms (calcium and redox; 'CaRe' status) sense temperature and identify genes and regulatory pathways likely to be involved in driving sexual development. We take advantage of the unique sex determining system of the model organism, Pogona vitticeps, to assess predictions of this hypothesis. P. vitticeps has ZZ male: ZW female sex chromosomes whose influence can be overridden in genetic males by high temperatures, causing male-to-female sex reversal. We compare a developmental transcriptome series of ZWf females and temperature sex reversed ZZf females. We demonstrate that early developmental cascades differ dramatically between genetically driven and thermally driven females, later converging to produce a common outcome (ovaries). We show that genes proposed as regulators of thermosensitive sex determination play a role in temperature sex reversal. Our study greatly advances the search for the mechanisms by which temperature determines sex.

History

Publication Date

15/04/2021

Journal

PLoS Genetics

Volume

17

Issue

4

Article Number

e1009465

Pagination

33p.

Publisher

Public Library of Science (PLOS)

ISSN

1553-7390

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The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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