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Treatment of Staphylococcus aureus skin infection in vivo using rifampicin loaded lipid nanoparticles

journal contribution
posted on 2021-01-12, 05:30 authored by A Walduck, P Sangwan, QA Vo, Julian RatcliffeJulian Ratcliffe, J White, Benjamin MuirBenjamin Muir, N Tran
© The Royal Society of Chemistry 2020. We have previously reported on a novel nanoparticle formulation that was effective at killingStaphylococcus aureus in vitro. Here, we report for the first time, the antibacterial effects of a lipidic nano-carrier containing rifampicin (NanoRIF) which can be used to successfully treat Methicillin-ResistantS. aureus(MRSA) infection at a reduced antibiotic dosage compared to the free drug in a skin wound model in mice. The formulation used contains the lipid monoolein, a cationic lipidN-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (DOTAP) and the antibiotic. We have shown that rifampicin-loaded nanoparticles are more effective at treating infection in the skin wound model than the antibiotic alone. Cryo-TEM was used to capture for the first time, interactions of the formed nanoparticles with the cell wall of an individual bacterium. Our data strongly indicate enhanced binding of these charged nanoparticles with the negatively charged bacterial membrane. The efficacy we have now observedin vivois of significant importance for the continued development of nanomedicine-based strategies to combat antibiotic resistant bacterial skin infections.


B. M. gratefully acknowledges funding through his CSIRO Office of the Chief Executive (OCE) Julius Career Award to conduct this research. N. T. is supported by a RMIT VC Research Fellowship. AW is supported by start-up funds from RMIT. The authors thank Mr Wil Gardner for assistance with rendering the cubic phase schematic image in Blender 2.81a.


Publication Date



RSC Advances: an international journal to further the chemical sciences






12p. (p. 33608-33619)





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