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Transcriptome and long noncoding RNA sequencing of three extracellular vesicle subtypes released from the human colon cancer LIM1863 cell line

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posted on 05.08.2021, 07:00 by Maoshan Chen, Rong Xu, H Ji, David GreeningDavid Greening, A Rai, K Izumikawa, H Ishikawa, N Takahashi, Richard SimpsonRichard Simpson
Previously we reported that LIM1863 colorectal cancer (CRC) cells secrete three distinct extracellular vesicle subtypes-two subpopulations of exosomes (apical EpCAM-Exos and basolateral A33-Exos) and shed microvesicles (sMVs)-with distinct protein and miRNA signatures. Here, we extend our omics approach to understand the fundamental role of LIM1863-derived EVs by performing a comprehensive analysis of their mRNAs and long non-coding RNAs (lncRNAs) using RNA-Seq. We show that 2,389 mRNAs, 317 pseudogene transcripts, 1,028 lncRNAs and 206 short non-coding RNAs selectively distributed to (i.e., are enriched in) LIM1863 EVs, relative to the parent cell. An Ensembl/UniProtKB analysis revealed 1,937 mRNAs encode canonical proteins, 348 isoforms (including splice-variant proteins), and 119 'missing proteins' (i.e., annotated in Ensembl but not UniProtKB). Further dissection of our protein/RNA data revealed that 6/151 observed RNA binding proteins have the potential to interact with ∼75% of EV-enriched RNAs. Intriguingly, the co-existence of U1 and U2 ribonucleoproteins and their cognate snRNAs in LIM1863 EVs suggests a possible association of CRC EVs with recipient cell splicing events. Our data reveal several potential lncRNA CRC biomarkers and novel splicing/fusion genes that, collectively, will advance our understanding of EV biology in CRC and accelerate the development of EV-based diagnostics and therapeutics.

Funding

RJS is supported by a Distinguished Professorship from La Trobe University and DWG by a Stone Fellowship (La Trobe University). MC, AR and RX are supported by La Trobe University Postgraduate Research Scholarships (LTUPRS). NT, KI, HI, are supported by Core Research for Evolutional Science and technology (CREST) from Japan Science and Technology Agency (JST).

History

Publication Date

05/12/2016

Journal

Scientific Reports

Volume

6

Issue

1

Article Number

38397

Pagination

14p.

Publisher

Springer Nature

ISSN

2045-2322

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