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Transcriptional and biochemical analyses of gibberellin expression and content in germinated barley grain
journal contributionposted on 19.01.2021, 00:51 by Natalie S Betts, Christoph Dockter, Oliver Berkowitz, Helen M Collins, Michelle Hooi, Qiongxian Lu, Rachel A Burton, Vincent Bulone, Birgitte Skadhauge, James Whelan, Geoffrey B Fincher
© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. Mobilization of reserves in germinated cereal grains is critical for early seedling vigour, global crop productivity, and hence food security. Gibberellins (GAs) are central to this process. We have developed a spatio-temporal model that describes the multifaceted mechanisms of GA regulation in germinated barley grain. The model was generated using RNA sequencing transcript data from tissues dissected from intact, germinated grain, which closely match measurements of GA hormones and their metabolites in those tissues. The data show that successful grain germination is underpinned by high concentrations of GA precursors in ungerminated grain, the use of independent metabolic pathways for the synthesis of several bioactive GAs during germination, and a capacity to abort bioactive GA biosynthesis. The most abundant bioactive form is GA1, which is synthesized in the scutellum as a glycosyl conjugate that diffuses to the aleurone, where it stimulates de novo synthesis of a GA3 conjugate and GA4. Synthesis of bioactive GAs in the aleurone provides a mechanism that ensures the hormonal signal is relayed from the scutellum to the distal tip of the grain. The transcript data set of 33 421 genes used to define GA metabolism is available as a resource to analyse other physiological processes in germinated grain.
We thank the Australian Research Council (LP160100700) and the Carlsberg Foundation (CF15-0452) for financial support, and Andrea Matros, Finn Lok, and Pai Rosager Pedas for helpful discussions. We also acknowledge the La Trobe University Genomics Platform for providing access to next-generation sequencing equipment, and Asha Haslem for technical assistance.
JournalJournal of Experimental Botany
Pagination15p. (p. 1870-1884)
PublisherOxford University Press (OUP)
Rights StatementThe Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.
Science & TechnologyLife Sciences & BiomedicinePlant SciencesAleuronegerminationgibberellic acidHordeum vulgareRNA-seqscutellumsignal transductionTANDEM MASS-SPECTROMETRYISOLATED ALEURONE LAYERSALPHA-AMYLASEABSCISIC-ACIDENT-KAURENEBIOSYNTHESISGENEVISUALIZATIONMETABOLISMSYNTHASEPlant Biology & Botany