1177133_SoaresdaCosta,T_2021.pdf (2.91 MB)
Towards novel herbicide modes of action by inhibiting lysine biosynthesis in plants
journal contributionposted on 2021-08-25, 01:26 authored by Tatiana Soares-da-CostaTatiana Soares-da-Costa, Cody HallCody Hall, S Panjikar, Jessica WyllieJessica Wyllie, Rebecca Christoff, S Bayat, Mark HulettMark Hulett, Belinda AbbottBelinda Abbott, Anthony GendallAnthony Gendall, Matthew PeruginiMatthew Perugini
Weeds are becoming increasingly resistant to our current herbicides, posing a significant threat to agricultural production. Therefore, new herbicides with novel modes of action are urgently needed. In this study, we exploited a novel herbicide target, dihydrodipicolinate synthase (DHDPS), which catalyses the first and rate-limiting step in lysine biosynthesis. The first class of plant DHDPS inhibitors with micromolar potency against Arabidopsis thaliana DHDPS were identified using a high throughput chemical screen. We determined that this class of inhibitors binds to a novel and unexplored pocket within DHDPS, which is highly conserved across plant species. The inhibitors also attenuated the germination and growth of A. thaliana seedlings and confirmed their pre-emergence herbicidal activity in soil-grown plants. These results provide proof-of-concept that lysine biosynthesis represents a promising target for the development of herbicides with a novel mode of action to tackle the global rise of herbicide resistant weeds.
National Health and Medical Research Council of Australia APP1091976 Tatiana P Soares da Costa Australian Research Council DE190100806 Tatiana P Soares da Costa Australian Research Council DP150103313 Santosh Panjikar Matthew A Perugini Australian Research Council IH180100006 Anthony R Gendall The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Article NumberARTN e69444
PublisherELIFE SCIENCES PUBLICATIONS LTD
Rights StatementThe Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.
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