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The synthesis and application of a colour-switch β-arylethenesulfonyl fluoride fluorescent probe in the detection of serum albumin

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Version 1 2023-08-25, 00:57
journal contribution
posted on 2023-08-25, 00:57 authored by Marie-Claire GielMarie-Claire Giel, Tze Cin Owyong, Yuning HongYuning Hong
Proteins play a pivotal role in regulating important physiological processes and serve as important biomarkers for many diseases. Herein, we present a new strategy for bovine serum albumin (BSA) detection using a novel colour-switch fluorescent probe CPV-ESF ((E)-2-(4-((Z)-1-cyano-2-(4-(diethylamino)phenyl)vinyl)phenyl)ethene-1-sulfonyl fluoride). CPV-ESF reacts with nucleophilic amino acids of BSA via 1,4-Michael addition click chemistry to create a covalently linked CPV-ESF:BSA complex, which can be easily detected by a fluorescence colour-switch response. The sensing mechanism, sensitivity and selectivity of CPV-ESF for BSA detection as well as its application for cell imaging have been investigated.

Funding

This work was supported by funding from the Australian Research Council (FT210100271) and Australia–China Science and Research Fund Joint Research Centre on Personal Health Technologies (ACSRF65777).

History

Publication Date

2022-11-22

Journal

Australian Journal of Chemistry

Volume

75

Issue

11

Pagination

7p. (p. 877-883)

Publisher

CSIRO

ISSN

0004-9425

Rights Statement

© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).

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