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The role of Arabidopsis ABA receptors from the PYR/PYL/RCAR family in stomatal acclimation and closure signal integration
journal contributionposted on 2021-01-12, 05:41 authored by Marcus Dittrich, Heike M Mueller, Hubert Bauer, Marta Peirats-LlobetMarta Peirats-Llobet, Pedro L Rodriguez, Christoph-Martin Geilfus, Sebastien Christian Carpentier, Khaled AS Al Rasheid, Hannes Kollist, Ebe Merilo, Johannes Herrmann, Tobias Müller, Peter Ache, Alistair M Hetherington, Rainer Hedrich
© 2019, The Author(s), under exclusive licence to Springer Nature Limited.
Stomata are microscopic pores found on the surfaces of leaves that act to control CO2 uptake and water loss. By integrating information derived from endogenous signals with cues from the surrounding environment, the guard cells, which surround the pore, ‘set’ the stomatal aperture to suit the prevailing conditions. Much research has concentrated on understanding the rapid intracellular changes that result in immediate changes to the stomatal aperture. In this study, we look instead at how stomata acclimate to longer timescale variations in their environment. We show that the closure-inducing signals abscisic acid (ABA), increased CO2, decreased relative air humidity and darkness each access a unique gene network made up of clusters (or modules) of common cellular processes. However, within these networks some gene clusters are shared amongst all four stimuli. All stimuli modulate the expression of members of the PYR/PYL/RCAR family of ABA receptors. However, they are modulated differentially in a stimulus-specific manner. Of the six members of the PYR/PYL/RCAR family expressed in guard cells, PYL2 is sufficient for guard cell ABA-induced responses, whereas in the responses to CO2, PYL4 and PYL5 are essential. Overall, our work shows the importance of ABA as a central regulator and integrator of long-term changes in stomatal behaviour, including sensitivity, elicited by external signals. Understanding this architecture may aid in breeding crops with improved water and nutrient efficiency.
Work in the Hedrich laboratory was supported by a grant from King Saud University Deanship for Scientific Research, International Research Group Programme (IRG14-22), Riyadh, Saudi Arabia, and that of the Rodriguez laboratory was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional and Consejo Superior de Investigaciones Cientificas (grants BIO2014 52537-R and BIO2017 82503-R, to P.L.R.). M.D. was supported by the CRC/Transregio124 - FungiNet funded by the Deutsche Forschungsgemeinschaft (project B2). A.M.H. acknowledges support from the UK BBSRC (grant no. BB/N001168/1). E.M. was supported by the Estonian Research Council (grant no. PUT1133).
Pagination10p. (p. 1002-1011)
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Science & TechnologyLife Sciences & BiomedicinePlant SciencesABSCISIC-ACIDGENETIC MANIPULATIONINTERACTION NETWORKSCARBON-DIOXIDECO2PHOTOSYNTHESISPHOSPHATASESPERCEPTIONMECHANISMSINHIBITIONArabidopsisAbscisic AcidIntracellular Signaling Peptides and ProteinsReceptors, Cell SurfaceArabidopsis ProteinsSignal TransductionPlant Stomata