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The mitochondrial LYR protein SDHAF1 is required for succinate dehydrogenase activity in Arabidopsis

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posted on 2022-06-21, 00:25 authored by Y Li, K Belt, SF Alqahtani, S Saha, R Fenske, O Van Aken, James WhelanJames Whelan, AH Millar, MW Murcha, S Huang
Succinate dehydrogenase (SDH, complex II), which plays an essential role in mitochondrial respiration and tricarboxylic acid metabolism, requires the assembly of eight nuclear-encoded subunits and the insertion of various cofactors. Here, we report on the characterization of an Arabidopsis thaliana leucine-tyrosine-arginine (LYR) protein family member SDHAF1, (At2g39725) is a factor required for SDH activity. SDHAF1 is located in mitochondria and can fully complement the yeast SDHAF1 deletion strain. Knockdown of SDHAF1 using RNA interference resulted in a decrease in seedling hypocotyl elongation and reduced SDH activity. Proteomic analyses revealed a decreased abundance of various SDH subunits and assembly factors. Protein interaction assays revealed that SDHAF1 can interact exclusively with the Fe-S cluster-containing subunit SDH2 and HSCB, a cochaperone involved in Fe-S cluster complex recruitment. Therefore, we propose that in Arabidopsis, SDHAF1 plays a role in the biogenesis of SDH2 to form the functional complex II, which is essential for mitochondrial respiration and metabolism.


KB was supported by a Scholarship for International Research Fees (SIRF) from the University of Western Australia. JW and MWM are funded by an Australian Research Council (ARC) grant (DP210103258).


Publication Date



The Plant Journal






14p. (p. 499-512)





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©2022 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.