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The mechanism of Pseudomonas aeruginosa outer membrane vesicle biogenesis determines their protein composition

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posted on 2024-05-15, 03:25 authored by Lauren ZavanLauren Zavan, H Fang, Ella JohnstonElla Johnston, C Whitchurch, David GreeningDavid Greening, Andrew HillAndrew Hill, Maria LiaskosMaria Liaskos
Gram-negative bacteria produce outer membrane vesicles (OMVs) and contain bacterial cargo including nucleic acids and proteins. The proteome of OMVs can be altered by various factors including bacterial growth stage, growth conditions, and environmental factors. However, it is currently unknown if the mechanism of OMV biogenesis can determine their proteome. In this study, we examined whether the mechanisms of OMV biogenesis influenced the production and protein composition of Pseudomonas aeruginosa OMVs. OMVs were isolated from three P. aeruginosa strains that produced OMVs either by budding alone, by explosive cell lysis, or by both budding and explosive cell lysis. We identified that the mechanism of OMV biogenesis dictated OMV quantity. Furthermore, a global proteomic analysis comparing the proteome of OMVs to their parent bacteria showed significant differences in the identification of proteins in bacteria and OMVs. Finally, we determined that the mechanism of OMV biogenesis influenced the protein composition of OMVs, as OMVs released by distinct mechanisms of biogenesis differed significantly from one another in their proteome and functional enrichment analysis. Overall, our findings reveal that the mechanism of OMV biogenesis is a main factor that determines the OMV proteome which may affect their subsequent biological functions.


This work was supported by the Australian Research Council (project: Maria Kaparakis-Liaskos, Andrew F. Hill) under grant DP190101655. Maria Kaparakis-Liaskos was supported by a veski Inspiring Women Fellowship. This work was supported by the LIMS Bioimaging Facility (La Trobe University), and the Defence Science Institute (Lauren Zavan, Maria Kaparakis-Liaskos), an initiative of the State Government of Victoria.


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© 2023 The Authors. Proteomics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.