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The formulation of bacteriophage in a semi solid preparation for control of Propionibacterium acnes growth

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posted on 2023-02-03, 00:21 authored by Teagan Brown, Steve PetrovskiSteve Petrovski, ZA Dyson, Robert SeviourRobert Seviour, Joseph TucciJoseph Tucci
Aims: To isolate and characterise phage which could lyse P. acnes and to formulate the phage into a delivery form for potential application in topical treatment of acne infection. Methods and Results: Using standard phage isolation techniques, ten phage capable of lysing P. acnes were isolated from human skin microflora. Their genomes showed high homology to previously reported P. acnes phage. These phage were formulated into cetomacrogol cream aqueous at a concentration of 2.5×108 PFU per gram, and shown to lyse underlying P. acnes cells grown as lawn cultures. These phage formulations remained active for at least 90 days when stored at four degrees Celsius in a light protected container. Conclusions: P. acnes phage formulated into cetomacrogol cream aqueous will lyse surrounding and underlying P. acnes bacteria, and are effective for at least 90 days if stored appropriately. Significance and Impact of the Study: There are few reports of phage formulation into semi solid preparations for application as phage therapy. The formulation method described here could potentially be applied topically to treat human acne infections. The potential exists for this model to be extended to other phage applied to treat other bacterial skin infections.

History

Publication Date

2016-03-10

Journal

PLoS One

Volume

11

Issue

3

Article Number

e0151184

Pagination

16p. (p. 1-16)

Publisher

PLOS

ISSN

1932-6203

Rights Statement

© 2016 Brown et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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