The first linkage map for Australo-Papuan Treefrogs (family: Pelodryadidae) reveals the sex-determination system of the Green-eyed Treefrog (Litoria serrata)

Bertola, LV; Hoskin, CJ; Jones, DB; Zenger, KR; McKnight, Donald; Higgie, M

doi:10.26181/24570553.v1

The first linkage map for Australo-Papuan Treefrogs (family: Pelodryadidae) reveals the sex-determination system of the Green-eyed Treefrog (Litoria serrata)

journal contribution

posted on 2023-11-15, 23:48 authored by LV Bertola, CJ Hoskin, DB Jones, KR Zenger, Donald McKnightDonald McKnight, M Higgie

Amphibians represent a useful taxon to study the evolution of sex determination because of their highly variable sex-determination systems. However, the sex-determination system for many amphibian families remains unknown, in part because of a lack of genomic resources. Here, using an F1 family of Green-eyed Treefrogs (Litoria serrata), we produce the first genetic linkage map for any Australo-Papuan Treefrogs (family: Pelodryadidae). The resulting linkage map contains 8662 SNPs across 13 linkage groups. Using an independent set of sexed adults, we identify a small region in linkage group 6 matching an XY sex-determination system. These results suggest Litoria serrata possesses a male heterogametic system, with a candidate sex-determination locus on linkage group 6. Furthermore, this linkage map represents the first genomic resource for Australo-Papuan Treefrogs, an ecologically diverse family of over 220 species.

Funding

This research was funded by an Australian Research Council DECRA Fellowship to MH (DE130100218), a Skyrail Rainforest Foundation Student Grant, and James Cook University research grants (to MH and CJH). This research is supported by an Australian Government Research Training Program (RTP) Scholarship granted to LVB at James Cook University.

History

Publication Date

2023-10-01

Journal

Heredity

Volume

131

Pagination

10p. (p. 263-272)

Publisher

Springer Nature

ISSN

0018-067X

Rights Statement

© The Author(s) 2023 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.