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Somatic proteome of Haemonchus contortus

Version 2 2023-03-29, 03:10
Version 1 2021-03-17, 03:52
journal contribution
posted on 2021-03-17, 03:52 authored by Tao Wang, Guangxu Ma, Ching-Seng Ang, Pasi K Korhonen, Rong Xu, Shuai Nie, Anson V Koehler, Richard J Simpson, David GreeningDavid Greening, Gavin E Reid, Nicholas A Williamson, Robin B Gasser
© 2019 Australian Society for Parasitology Currently, there is a dearth of proteomic data to underpin fundamental investigations of parasites and parasitism at the molecular level. Here, using a high throughput LC–MS/MS-based approach, we undertook the first reported comprehensive, large-scale proteomic investigation of the barber's pole worm (Haemonchus contortus) – one of the most important parasitic nematodes of livestock animals worldwide. In total, 2487 unique H. contortus proteins representing different developmental stages/sexes (i.e. eggs, L3s and L4s, female (Af) and male (Am) adults) were identified and quantified with high confidence. Bioinformatic analyses of this proteome revealed substantial alterations in protein profiles during the life cycle, particularly in the transition from the free-living to the parasitic phase, and key groups of proteins involved specifically in feeding, digestion, metabolism, development, parasite-host interactions (including immunomodulation), structural remodelling of the body wall and adaptive processes during parasitism. This proteomic data set will facilitate future molecular, biochemical and physiological investigations of H. contortus and related nematodes, and the discovery of novel intervention targets against haemonchosis.


Funding from the National Health and Medical Research Council (NHMRC) of Australia, the Australian Research Council, Melbourne Water Corporation, Australia, and The University of Melbourne (BIP), Australia, is gratefully acknowledged (R.B.G. et al.) as is support from the Melbourne Bioinformatics Platform, Australia. P.K.K. holds an NHMRC Early Career Researcher Fellowship (ECRF). We are grateful to Mr Qiang Huang for his support in the preparation of Fig. 2. Funding bodies played no role in the design of the study or collection, analysis or interpretation of data, or in the writing of the manuscript.


Publication Date



International Journal for Parasitology






10p. (p. 311-320)





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