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1038530_Singhal,G_2021.pdf (1.75 MB)

Short-term environmental enrichment is a stronger modulator of brain glial cells and cervical lymph node t cell subtypes than exercise or combined exercise and enrichment

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posted on 2021-05-19, 02:23 authored by G Singhal, Julie Morgan, F Corrigan, C Toben, MC Jawahar, Emily JaehneEmily Jaehne, J Manavis, AJ Hannan, BT Baune
Physical exercise (PE) and environmental enrichment (EE) can modulate immunity. However, the differential effects of short-term PE, EE, and PE + EE on neuroimmune mechanisms during normal aging has not been elucidated. Hence, a cohort of 3-, 8-, and 13-month-old immunologically unchallenged C57BL/6 wild-type mice were randomly assigned to either Control, PE, EE, or PE + EE groups and provided with either no treatment, a running wheel, a variety of plastic and wooden objects alone or in combination with a running wheel for seven weeks, respectively. Immunohistochemistry and 8-color flow cytometry were used to determine the numbers of dentate gyrus glial cells, and the proportions of CD4 and CD8 T cell numbers and their subsets from cervical lymph nodes, respectively. An increase in the number of IBA1 microglia in the dentate gyrus at 5 and 10 months was observed after EE, while PE and PE + EE increased it only at 10 months. No change in astroglia number in comparison to controls were observed in any of the treatment groups. Also, all treatments induced significant differences in the proportion of specific T cell subsets, i.e., CD4 and CD8 T naïve (T ), central memory (T ), and effector memory (T ) cells. Our results suggest that in the short-term, EE is a stronger modulator of microglial and peripheral T cell subset numbers than PE and PE + EE, and the combination of short-term PE and EE has no additive effects. + + + + + N CM EM


Open Access funding provided by Projekt DEAL. The presented work is supported by the National Health and Medical Research Council Australia (APP 1043771 to BTB). AJH is an NHMRC Principal Research Fellow. Many thanks to the University of Adelaide for giving me the fantastic opportunity of working in one of the world's best facilities alongside world-renowned researchers; the National Health and Medical Research Council (NHMRC) for the financial support of this project without which this work would not have been possible; Ms. Katherine Pilkington and Ms. Rebecca Bassmann for their guidance with Fluorescent Activated Cell Sorting analysis; and Ms. Pacita Wissell and staff at the Laboratory Animal Services, The University of Adelaide, for their assistance with mouse husbandry work.


Publication Date



Cellular and Molecular Neurobiology






18p. (p. 469-486)





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