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Sex-specific splicing of Z- and W-borne nr5a1 alleles suggests sex determination is controlled by chromosome conformation

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posted on 2022-02-11, 04:42 authored by X Zhang, S Wagner, CE Holleley, JE Deakin, K Matsubara, IW Deveson, D O'Meally, HR Patel, T Ezaz, Z Li, C Wang, M Edwards, Jennifer GravesJennifer Graves, A Georges
Pogona vitticeps has female heterogamety (ZZ/ZW), but the master sex-determining gene is unknown, as it is for all reptiles. We show that nr5a1 (Nuclear Receptor Subfamily 5 Group A Member 1), a gene that is essential in mammalian sex determination, has alleles on the Z and W chromosomes (Z-nr5a1 and W-nr5a1), which are both expressed and can recombine. Three transcript isoforms of Z-nr5a1 were detected in gonads of adult ZZ males, two of which encode a functional protein. However, ZW females produced 16 isoforms, most of which contained premature stop codons. The array of transcripts produced by the W-borne allele (W-nr5a1) is likely to produce truncated polypeptides that contain a structurally normal DNA-binding domain and could act as a competitive inhibitor to the full-length intact protein. We hypothesize that an altered configuration of the W chromosome affects the conformation of the primary transcript generating inhibitory W-borne isoforms that suppress testis determination. Under this hypothesis, the genetic sex determination (GSD) system of P. vitticeps is a W-borne dominant female-determining gene that may be controlled epigenetically.


Publication Date



Proceedings of the National Academy of Sciences of the United States of America






9 p.


Proceedings of the National Academy of Sciences



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Copyright © 2022 the Author(s). Published by PNAS. The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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