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Secreted midbody remnants are a class of extracellular vesicles molecularly distinct from exosomes and microparticles

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journal contribution
posted on 14.05.2021, 04:41 by A Rai, David Greening, Rong Xu, Maoshan Chen, Wittaya Suwakulsiri, Richard Simpson
During the final stages of cell division, newly-formed daughter cells remain connected by a thin intercellular bridge containing the midbody (MB), a microtubule-rich organelle responsible for cytokinetic abscission. Following cell division the MB is asymmetrically inherited by one daughter cell where it persists as a midbody remnant (MB-R). Accumulating evidence shows MB-Rs are secreted (sMB-Rs) into the extracellular medium and engulfed by neighbouring non-sister cells. While much is known about intracellular MB-Rs, sMB-Rs are poorly understood. Here, we report the large-scale purification and biochemical characterisation of sMB-Rs released from colon cancer cells, including profiling of their proteome using mass spectrometry. We show sMB-Rs are an abundant class of membrane-encapsulated extracellular vesicle (200-600 nm) enriched in core cytokinetic proteins and molecularly distinct from exosomes and microparticles. Functional dissection of sMB-Rs demonstrated that they are engulfed by, and accumulate in, quiescent fibroblasts where they promote cellular transformation and an invasive phenotype.

Funding

A.R., D.W.G., M.C., R.X., W.S., and R.J.S. acknowledge funding support from La Trobe University, Melbourne, Australia. The authors thank Dr. Jacqueline Orian (La Trobe University) for assistance with immunohistochemistry.

History

Publication Date

01/12/2021

Journal

Communications Biology

Volume

4

Issue

1

Article Number

ARTN 400

Pagination

12p.

Publisher

NATURE RESEARCH

ISSN

2399-3642

Rights Statement

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