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Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with Na+ accumulation in leaves of chickpea (Cicer arietinum L.)
journal contributionposted on 2020-11-19, 05:38 authored by C Sweetman, G Khassanova, TK Miller, NJ Booth, A Kurishbayev, S Jatayev, NK Gupta, P Langridge, CLD Jenkins, KL Soole, David DayDavid Day, Y Shavrukov
© 2020, The Author(s). Background: Chickpea is an important legume and is moderately tolerant to salinity stress during the growing season. However, the level and mechanisms for salinity tolerance can vary among accessions and cultivars. A large family of CaRab-GTP genes, previously identified in chickpea, is homologous to intracellular vesicle trafficking superfamily genes that play essential roles in response to salinity stress in plants. Results: To determine which of the gene family members are involved in the chickpea salt response, plants from six selected chickpea accessions (Genesis 836, Hattrick, ICC12726, Rupali, Slasher and Yubileiny) were exposed to salinity stress and expression profiles resolved for the major CaRab-GTP gene clades after 5, 9 and 15 days of salt exposure. Gene clade expression profiles (using degenerate primers targeting all members of each clade) were tested for their relationship to salinity tolerance measures, namely plant biomass and Na+ accumulation. Transcripts representing 11 out of the 13 CaRab clades could be detected by RT-PCR, but only six (CaRabA2, −B, −C, −D, −E and −H) could be quantified using qRT-PCR due to low expression levels or poor amplification efficiency of the degenerate primers for clades containing several gene members. Expression profiles of three gene clades, CaRabB, −D and −E, were very similar across all six chickpea accessions, showing a strongly coordinated network. Salt-induced enhancement of CaRabA2 expression at 15 days showed a very strong positive correlation (R2 = 0.905) with Na+ accumulation in leaves. However, salinity tolerance estimated as relative plant biomass production compared to controls, did not correlate with Na+ accumulation in leaves, nor with expression profiles of any of the investigated CaRab-GTP genes. Conclusion: A coordinated network of CaRab-GTP genes, which are likely involved in intracellular trafficking, are important for the salinity stress response of chickpea plants.
This study was supported by an Australian Research Council/Grains Research and Development Corporation, Industrial Transformation Research Hub grant, IH140100013 (DD) and by the Ministry of Education and Science, Kazakhstan, Research program BR05236500 (SJ). Publication costs are funded by both grant and program. The funders had no role in study design, data collection analysis and interpretation, decision to publish, or preparation of the manuscript.
JournalBMC Plant Biology
Article NumberARTN 183
Rights StatementThe Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.
Science & TechnologyLife Sciences & BiomedicinePlant SciencesChickpeaGene expressionRab-GTP genesSalt stressSalinityVesicle traffickingGDP-DISSOCIATION INHIBITORVACUOLAR TRAFFICKINGBINDING PROTEINSALINITY TOLERANCESODIUM EXCLUSIONRAB GTPASEMEMBRANE TRAFFICKINGSTRESS TOLERANCEOVEREXPRESSIONFAMILYPlant Biology & Botany