Saccharococcus caldoxylosilyticus sp. nov., an obligately thermophilic, xylose-utilizing, endospore-forming bacterium

La Trobe University Faculty of Science, Technology and Engineering Murray Darling Freshwater Research Centre

MDFRC item.

Several closely related, xylanolytic, thermophilic bacilli were isolated from local soils on xylose-containing minimal medium. On the basis of morphology and biochemical characteristics. one of the isolates, designated strain S1812(T) (T = type strain), was studied further. Strain S1812(T) was a xylanolytic, sporulating, Gram-positive, rod-shaped bacterium. Its Cram-positive nature was confirmed by electron microscopic examination of thin sections of the cells. The isolate was a thermophilic (optimum temperature for growth, 65 degrees C), facultative anaerobe that grew on a wide range of carbon sources including glucose, lactose, starch and xylose. It expressed high levels of both xylose isomerase and xylulokinase on xylose and also on glucose. The DNA G+C content was 44 mol%, rRNA gene sequence analysis placed strain S1812T in Bacillus cluster 5; it was more closely related to Saccharococcus thermophillus than to thermophilic Bacillus species. DNA-DNA hybridization also indicated its close relationship to 5. thermophilus. Based on the evidence presented, it is proposed that strain S1812(T) be designated Saccharococcus caldoxylosilyticus sp. nov. Strain S1812(T) is the type strain (= ATCC 700356(T) = DSM 97-987(T)).