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SARS-CoV-2 Mpro responds to oxidation by forming disulfide and NOS/SONOS bonds

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posted on 2024-05-20, 01:18 authored by Patrick YA Reinke, Robin Schubert, Dominik Oberthür, Marina Galchenkova, Aida Rahmani Mashhour, Sebastian Günther, Anaïs Chretien, Adam Round, Brandon Charles Seychell, Brenna Norton-Baker, Chan Kim, Christina Schmidt, Faisal HM Koua, Alexandra Tolstikova, Wiebke Ewert, Gisel Esperanza Peña Murillo, Grant Mills, Henry Kirkwood, Hévila Brognaro, Huijong Han, Jayanath Koliyadu, Joachim Schulz, Johan Bielecki, Julia Lieske, Julia Maracke, Juraj Knoska, Kristina Lorenzen, Lea Brings, Marcin Sikorski, Marco Kloos, Mohammad Vakili, Patrik Vagovic, Philipp Middendorf, Raphael de Wijn, Richard Bean, Romain Letrun, Seonghyun Han, Sven Falke, Tian Geng, Tokushi Sato, Vasundara Srinivasan, Yoonhee Kim, Oleksandr M Yefanov, Luca Gelisio, Tobias Beck, Andrew S Doré, Adrian MancusoAdrian Mancuso, Christian Betzel, Saša Bajt, Lars Redecke, Henry N Chapman, Alke Meents, Dušan Turk, Winfried Hinrichs, Thomas J Lane

Abstract: The main protease (Mpro) of SARS-CoV-2 is critical for viral function and a key drug target. Mpro is only active when reduced; turnover ceases upon oxidation but is restored by re-reduction. This suggests the system has evolved to survive periods in an oxidative environment, but the mechanism of this protection has not been confirmed. Here, we report a crystal structure of oxidized Mpro showing a disulfide bond between the active site cysteine, C145, and a distal cysteine, C117. Previous work proposed this disulfide provides the mechanism of protection from irreversible oxidation. Mpro forms an obligate homodimer, and the C117-C145 structure shows disruption of interactions bridging the dimer interface, implying a correlation between oxidation and dimerization. We confirm dimer stability is weakened in solution upon oxidation. Finally, we observe the protein's crystallization behavior is linked to its redox state. Oxidized Mpro spontaneously forms a distinct, more loosely packed lattice. Seeding with crystals of this lattice yields a structure with an oxidation pattern incorporating one cysteine-lysine-cysteine (SONOS) and two lysine-cysteine (NOS) bridges. These structures further our understanding of the oxidative regulation of Mpro and the crystallization conditions necessary to study this structurally.

History

Publication Date

2024-05-07

Journal

Nature Communications

Volume

15

Issue

1

Article Number

3827

Pagination

10p.

Publisher

Springer Nature

ISSN

2041-1723

Rights Statement

© The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.