S-nitrosylation and S-glutathionylation of Cys134 on troponin I have opposing competitive actions on Ca2+ sensitivity in rat fast-twitch muscle fibers
journal contributionposted on 2021-08-12, 06:32 authored by Travis DutkaTravis Dutka, JP Mollica, CR Lamboley, VC Weerakkody, David GreeningDavid Greening, Giuseppe PosterinoGiuseppe Posterino, Robyn MurphyRobyn Murphy, Graham LambGraham Lamb
Nitric oxide is generated in skeletal muscle with activity and decreases Ca2+ sensitivity of the contractile apparatus, putatively by S-nitrosylation of an unidentified protein. We investigated the mechanistic basis of this effect and its relationship to the oxidation-induced increase in Ca2+ sensitivity in mammalian fast-twitch (FT) fibers mediated by S-glutathionylation of Cys134 on fast troponin I (TnIf). Force-[Ca2+] characteristics of the contractile apparatus in mechanically skinned fibers were assessed by direct activation with heavily Ca2+-buffered solutions. Treatment with S-nitrosylating agents, S-nitrosoglutathione (GSNO) or S-nitroso-N-acetyl-penicillamine (SNAP), decreased pCa50 ( = −log10 [Ca2+] at half-maximal activation) by ~-0.07 pCa units in rat and human FT fibers without affecting maximum force, but had no effect on rat and human slow-twitch fibers or toad or chicken FT fibers, which all lack Cys134. The Ca2+ sensitivity decrease was 1) fully reversed with dithiothreitol or reduced glutathione, 2) at least partially reversed with ascorbate, indicative of involvement of S-nitrosylation, and 3) irreversibly blocked by low concentration of the alkylating agent, N-ethylmaleimide (NEM). The biotin-switch assay showed that both GSNO and SNAP treatments caused S-nitrosylation of TnIf. S-glutathionylation pretreatment blocked the effects of S-nitrosylation on Ca2+ sensitivity, and vice-versa. S-nitrosylation pretreatment prevented NEM from irreversibly blocking S-glutathionylation of TnIf and its effects on Ca2+ sensitivity, and likewise S-glutathionylation pretreatment prevented NEM block of S-nitrosylation. Following substitution of TnIf into rat slow-twitch fibers, S-nitrosylation treatment caused decreased Ca2+ sensitivity. These findings demonstrate that S-nitrosylation and S-glutathionylation exert opposing effects on Ca2+ sensitivity in mammalian FT muscle fibers, mediated by competitive actions on Cys134 of TnIf.
JournalAmerican Journal of Physiology: Cell Physiology
Pagination12p. (p. C316-C327)
PublisherAmerican Physiological Society
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Science & TechnologyLife Sciences & BiomedicineCell BiologyPhysiologymuscle fatigueskinned muscle fibercontractile apparatusoxidationNITRIC-OXIDECONTRACTILE APPARATUSSARCOPLASMIC-RETICULUMSULFHYDRYL-GROUPSPROTEIN FUNCTIONCALCIUMDYSFUNCTIONFORCEPEROXYNITRITEMODULATIONMuscle Fibers, Fast-TwitchCells, CulturedAnimalsChickensHumansRatsRats, Long-EvansRats, Sprague-DawleyCalciumNitric OxideCysteineGlutathioneTroponin ICalcium SignalingSpecies SpecificityBinding SitesProtein BindingIsometric ContractionMaleYoung Adult