Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)

Smith, Reannon; Sawbridge, Tim; Mann, Ross; Kaur, Jatinder; May, Tom W.; Edwards, Jacqueline

doi:10.1371/journal.pone.0232535

Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)

journal contribution

posted on 2025-10-13, 03:07 authored by Reannon Smith, Tim SawbridgeTim Sawbridge, Ross Mann, Jatinder KaurJatinder Kaur, Tom W. May, Jacqueline Edwards

<p dir="ltr">The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two library preparation kits and found the combination of the E.Z.N.A.® Forensic DNA kit for DNA extraction and the NuGen Ovation® Ultralow System library preparation kit was the most suitable for this purpose.</p>

History

Publication Date

2020-05-13

Journal

PLoS One

Volume

15

Issue

5

Article Number

e0232535

Pagination

21p.

Publisher

Public Library of Science

ISSN

1932-6203

Rights Statement

© 2020 Smith et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.