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Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis

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posted on 2023-02-01, 23:06 authored by N Dominado, John La-MarcaJohn La-Marca, NA Siddall, J Heaney, M Tran, Y Cai, F Yu, H Wang, Wayne Somers, LM Quinn, GR Hime

The Drosophila testis has been fundamental to understanding how stem cells interact with their endogenous microenvironment, or niche, to control organ growth in vivo. Here, we report the identification of two independent alleles for the highly conserved tumor suppressor gene, Retinoblastoma-family protein (Rbf), in a screen for testis phenotypes in X chromosome third-instar lethal alleles. Rbf mutant alleles exhibit overproliferation of spermatogonial cells, which is phenocopied by the molecularly characterized Rbf11 null allele. We demonstrate that Rbf promotes cell-cycle exit and differentiation of the somatic and germline stem cells of the testes. Intriguingly, depletion of Rbf specifically in the germline does not disrupt stem cell differentiation, rather Rbf loss of function in the somatic lineage drives overproliferation and differentiation defects in both lineages. Together our observations suggest that Rbf in the somatic lineage controls germline stem cell renewal and differentiation non-autonomously via essential roles in the microenvironment of the germline lineage.

Funding

This work was funded by NHMRC Project grants to G.H. (APP1048110) and L.M.Q. (APP628414) and an NHMRC postdoctoral fellowship to W.G.S.

History

Publication Date

2016-12-13

Journal

Stem Cell Reports

Volume

7

Issue

6

Pagination

12p. (p. 1152-1163)

Publisher

Elsevier

ISSN

2213-6711

Rights Statement

© 2016 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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