Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis

Cullinane, Meabh; Scofield, L; Murray, GL; Payne, MS; Bennett, CM; Garland, SM; Amir, Lisa

doi:10.26181/21692327.v1

Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis

journal contribution

posted on 2022-12-15, 00:38 authored by Meabh CullinaneMeabh Cullinane, L Scofield, GL Murray, MS Payne, CM Bennett, SM Garland, Lisa AmirLisa Amir

Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase-negative staphylococci (CoNS) are a possible alternative pathogen. This case-control study examined the role of CoNS in mastitis using isolates cultured from breast milk of 20 women with mastitis and 16 women without mastitis. Gene sequencing determined bacterial species, and random amplified polymorphic DNA (RAPD) analysis investigated strain-level variation. The majority of CoNS isolates were Staphylococcus epidermidis (182/199; 91%). RAPD analysis identified 33 unique S. epidermidis profiles, with no specific profile associated with mastitis cases.

History

Publication Date

2022-08-01

Journal

Australian and New Zealand Journal of Obstetrics and Gynaecology

Volume

62

Issue

4

Pagination

(p. 605-609)

Publisher

Wiley

ISSN

0004-8666

Rights Statement

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. © 2022 The Authors. Australian and New Zealand Journal of Obstetrics and Gynaecology published by John Wiley & Sons Australia, Ltd on behalf of Royal Australian and New Zealand College of Obstetricians and Gynaecologists.