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Quantification of plasma kynurenine metabolites following one bout of sprint interval exercise

journal contribution
posted on 07.01.2021, 05:36 by A Trepci, S Imbeault, Victoria Wyckelsma, H Westerblad, S Hermansson, DC Andersson, F Piehl, T Venckunas, M Brazaitis, S Kamandulis, L Brundin, S Erhardt, L Schwieler
© The Author(s) 2020. The kynurenine pathway of tryptophan degradation produces several neuroactive metabolites suggested to be involved in a wide variety of diseases and disorders, however, technical challenges in reliably detecting these metabolites hampers cross-comparisons. The main objective of this study was to develop an accurate, robust and precise bioanalytical method for simultaneous quantification of ten plasma kynurenine metabolites. As a secondary aim, we applied this method on blood samples taken from healthy subjects conducting 1 session of sprint interval exercise (SIE). It is well accepted that physical exercise is associated with health benefits and reduces risks of psychiatric illness, diabetes, cancer and cardiovascular disease, but also influences the peripheral and central concentrations of kynurenines. In line with this, we found that in healthy old adults (n = 10; mean age 64 years), levels of kynurenine increased 1 hour (P =.03) after SIE, while kynurenic acid (KYNA) concentrations were elevated after 24 hours (P =.02). In contrast, no significant changes after exercise were seen in young adults (n = 10; mean age 24 years). In conclusion, the described method performs well in reliably detecting all the analyzed metabolites in plasma samples. Furthermore, we also detected an age-dependent effect on the degree by which a single intense training session affects kynurenine metabolite levels.

History

Publication Date

01/01/2020

Journal

International Journal of Tryptophan Research

Volume

13

Pagination

12p. (p. 1-12)

Publisher

SAGE

ISSN

1178-6469

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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