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Protocol for using artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins

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posted on 30.03.2022, 04:23 authored by Zelun Zhi, X Ma, C Zhou, Adam MechlerAdam Mechler, S Zhang, P Liu
Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins. Then we describe approaches to measure the number density of perilipin 2 on natural lipid droplets, construct artificial lipid droplets, and determine the binding affinity of perilipin 2 on artificial lipid droplets. This protocol can be adapted to determine the binding properties of various lipid droplet-associated proteins. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021).

History

Publication Date

18/03/2022

Journal

STAR Protocols

Volume

3

Issue

1

Article Number

101214

Pagination

29p.

Publisher

Cell Press

ISSN

2666-1667

Rights Statement

ª 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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