posted on 2022-03-30, 04:23authored byZelun Zhi, X Ma, C Zhou, Adam MechlerAdam Mechler, S Zhang, P Liu
Here, we present a protocol to construct artificial lipid droplets to study the binding affinity of lipid droplet-associated proteins. We provide procedures to construct adiposomes and prepare recombinant lipid droplet-associated proteins. Then we describe approaches to measure the number density of perilipin 2 on natural lipid droplets, construct artificial lipid droplets, and determine the binding affinity of perilipin 2 on artificial lipid droplets. This protocol can be adapted to determine the binding properties of various lipid droplet-associated proteins. For complete details on the use and execution of this protocol, please refer to Ma et al. (2021).
History
Publication Date
2022-03-18
Journal
STAR Protocols
Volume
3
Issue
1
Article Number
101214
Pagination
29p.
Publisher
Cell Press
ISSN
2666-1667
Rights Statement
ª 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).