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Protocol for generation of human peptide-specific primary CD8+ T cell lines

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posted on 2022-10-04, 04:26 authored by Emma GrantEmma Grant, Stephanie GrasStephanie Gras

Directly ex vivo, peptide-specific CD8+ T cells are present at relatively low frequency and are typically in a resting state. This protocol details the expansion of memory peptide-specific CD8+ T cells by in vitro stimulation, which can be subsequently characterized using a range of assays including tetramer staining and intracellular cytokine staining. 

History

Publication Date

2022-09-16

Journal

STAR Protocols

Volume

3

Issue

3

Article Number

101590

Pagination

14p.

Publisher

Elsevier

ISSN

2666-1667

Rights Statement

© 2022 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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