Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

Chatzileontiadou, Dimitra; Szeto, Christopher; Jayasinghe, D; Gras, Stephanie

doi:10.26181/6107ad23ead2b

1169773_Chatzileontiadou,D_2021.pdf (2.41 MB)

Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

journal contribution

posted on 2021-08-02, 08:30 authored by Dimitra ChatzileontiadouDimitra Chatzileontiadou, Christopher SzetoChristopher Szeto, D Jayasinghe, Stephanie GrasStephanie Gras

Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).

History

Publication Date

2021-09-17

Journal

STAR Protocols

Volume

2

Issue

3

Article Number

100635

Pagination

16pp.

Publisher

Elsevier BV

ISSN

2666-1667

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