1169773_Chatzileontiadou,D_2021.pdf (2.41 MB)
Download fileProtein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
journal contribution
posted on 2021-08-02, 08:30 authored by Dimitra ChatzileontiadouDimitra Chatzileontiadou, Christopher SzetoChristopher Szeto, D Jayasinghe, Stephanie GrasStephanie GrasUnderstanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).