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Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

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posted on 2021-08-02, 08:30 authored by Dimitra ChatzileontiadouDimitra Chatzileontiadou, Christopher SzetoChristopher Szeto, D Jayasinghe, Stephanie GrasStephanie Gras
Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).

History

Publication Date

2021-09-17

Journal

STAR Protocols

Volume

2

Issue

3

Article Number

100635

Pagination

16pp.

Publisher

Elsevier BV

ISSN

2666-1667

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The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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