La Trobe
Post-Translational and Transcriptional Dynamics – Regulating Extracellular Vesicle Biology.pdf (59.75 kB)

Post-translational and transcriptional dynamics–regulating  extracellular vesicle biology

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journal contribution
posted on 13.08.2021, 01:36 by Bethany ClaridgeBethany Claridge, K Kastaniegaard, Allan Stensballe, David GreeningDavid Greening
Introduction: Extracellular vesicles (EVs) are secreted into their extracellular environment, contain a specific repertoire of cellular cargo, and represent a novel vehicle for cell–cell communication. Protein post-translational modifications (PTMs) are emerging as major effectors of EV biology and function, and in turn, regulate cellular signaling. Areas covered: Discovery and investigation of PTMs such as methylation, glycosylation, acetylation, phosphorylation, sumoylation, and many others has established fundamental roles for PTMs within EVs and associated EV function. The application of enrichment strategies for modifications, high-resolution quantitative mass spectrometry-based proteomics, and improved technological approaches have provided key insights into identification and characterization of EV-based PTMs. Recently, an overwhelming appreciation for the diversity of modifications, including post-transcriptional modifications, dynamic roles of these modifications, and their emerging interplay, including protein–protein, protein–lipid, protein–RNA, and variable RNA modifications, is emerging. At a cellular level, such interplay is essential for gene expression/genome organization, protein function and localization, RNA metabolism, cell division, and cell signaling. Expert commentary: The understanding of these modifications and interactions will provide strategies toward how distinct cargo is localized, sorted, and delivered through EVs to mediate intercellular function, with further understanding of such modifications and intermolecular interactions will provide advances in EV-based therapeutic strategies.

Funding

This work was supported, in part, by the La Trobe University Stone Fellowship (D.W.G), the Australian National Health and Medical Research Council (Project: 1139489 and 1141946, D.W.G), and The Lundbeck Foundation grant (R247-2017-239; AS).

History

Publication Date

01/01/2019

Journal

Expert Review of Proteomics

Volume

16

Issue

1

Pagination

15p. (p. 17-31)

Publisher

Taylor and Francis

ISSN

1478-9450

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