16NiuPlosOne Physiological and Transcriptome Responses to Combinations of Elevated CO2 and Magnesium.pdf (7.47 MB)
Physiological and transcriptome responses to combinations of elevated CO2 and magnesium in Arabidopsis thaliana
journal contributionposted on 2021-01-05, 05:45 authored by Y Niu, GJ Ahammed, Caixian TangCaixian Tang, L Guo, J Yu
© 2016 Niu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The unprecedented rise in atmospheric CO2 concentration and injudicious fertilization or heterogeneous distribution of Mg in the soil warrant further research to understand the synergistic and holistic mechanisms involved in the plant growth regulation. This study investigated the influence of elevated CO2 (800 μL L-1) on physiological and transcriptomic profiles in Arabidopsis cultured in hydroponic media treated with 1 μM (low), 1000 μM (normal) and 10000 μM (high) Mg2+. Following 7-d treatment, elevated CO2 increased the shoot growth and chlorophyll content under both low and normal Mg supply, whereas root growth was improved exclusively under normal Mg nutrition. Notably, the effect of elevated CO2 on mineral homeostasis in both shoots and roots was less than that of Mg supply. Irrespective of CO2 treatment, high Mg increased number ofyoung leaf but decreased root growth and absorption of P, K, Ca, Fe and Mn whereas low Mg increased the concentration of P, K, Ca and Fe in leaves. Transcriptomics results showed that elevated CO2 decreased the expression of genes related to cell redox homeostasis, cadmium response, and lipid localization, but enhanced signal transduction, protein phosphorylation, NBS-LRR disease resistance proteins and subsequently programmed cell death in low-Mg shoots. By comparison, elevated CO2 enhanced the response of lipid localization (mainly LTP transfer protein/protease inhibitor), endomembrane system, heme binding and cell wall modification in high-Mg roots. Some of these transcriptomic results are substantially in accordance with our physiological and/or biochemical analysis. The present findings broaden our current understanding on the interactive effect of elevated CO2 and Mg levels in the Arabidopsis, which may help to design the novel metabolic engineering strategies to cope with Mg deficiency/excess in crops under elevated CO2.
This work was supported by grants of the China Postdoctoral Science Foundation (517000-X91403, http://jj.chinapostdoctor.org.cn/V1/Program1/Default.aspx), the National Natural Science Foundation of China (31550110201, http://www.nsfc.gov.cn/), the Zhejiang Provincial priority projects in forestry sciences (KF201310 and KF201311, http://kjc2.zafu.edu.cn/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.We thank anonymous reviewers for their constructive comments on the manuscript. We thank Hangzhou Guhe Information and Technology Co., Ltd for the help with RNA-seq sequencing and bioinformatics analysis. This work was financially supported by Grants of the China Postdoctoral Science Foundation (517000-X91403, http://jj.chinapostdoctor.org.cn/V1/Program1/Default.aspx), the National Natural Science Foundation of China (31550110201, http://www.nsfc.gov.cn/) and the Zhejiang Provincial priority projects in forestry sciences (KF201310 and KF201311).
PublisherPublic Library of Science
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Science & TechnologyMultidisciplinary SciencesScience & Technology - Other TopicsATMOSPHERIC CARBON-DIOXIDEENZYME-ACTIVITIESPHOTOSYSTEM-IIBEAN-PLANTSROOT HAIRSL. HEYNHDEFICIENCYGENETRANSPORTEREXPRESSIONCell WallArabidopsisPlant ShootsPlant RootsCarbon DioxideMagnesiumMineralsRNA, MessengerCluster AnalysisReproducibility of ResultsGene Expression ProfilingGene Expression Regulation, PlantHomeostasisGenes, PlantReal-Time Polymerase Chain ReactionTranscriptomeGeneral Science & Technology