La Trobe
1093657_Li,J_2020.pdf (4.52 MB)
Download file

Partial pathogenicity chromosomes in Fusarium oxysporum are sufficient to cause disease and can be horizontally transferred

Download (4.52 MB)
journal contribution
posted on 31.03.2021, 22:28 by J Li, L Fokkens, Lee James CONNEELYLee James CONNEELY, M Rep
© 2020 The Author. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. In Fusarium oxysporum f.sp. lycopersici, all effector genes reported so far – also called SIX genes – are located on a single accessory chromosome which is required for pathogenicity and can also be horizontally transferred to another strain. To narrow down the minimal region required for virulence, we selected partial pathogenicity chromosome deletion strains by fluorescence-assisted cell sorting of a strain in which the two arms of the pathogenicity chromosome were labelled with GFP and RFP respectively. By testing the virulence of these deletion mutants, we show that the complete long arm and part of the short arm of the pathogenicity chromosome are not required for virulence. In addition, we demonstrate that smaller versions of the pathogenicity chromosome can also be transferred to a non-pathogenic strain and they are sufficient to turn the non-pathogen into a pathogen. Surprisingly, originally non-pathogenic strains that had received a smaller version of the pathogenicity chromosome were much more aggressive than recipients with a complete pathogenicity chromosome. Whole genome sequencing analysis revealed that partial deletions of the pathogenicity chromosome occurred mainly close to repeats, and that spontaneous duplication of sequences in accessory regions is frequent both in chromosome deletion strains and in horizontal transfer strains.

History

Publication Date

01/12/2020

Journal

Environmental Microbiology

Volume

22

Issue

12

Pagination

20p. (p. 4985-5004)

Publisher

Wiley

ISSN

1462-2912

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.