1190525_Li,C_2022.pdf (2.62 MB)
Optimised Method for Short-Chain Fatty Acid Profiling of Bovine Milk and Serum
journal contributionposted on 2022-07-29, 00:03 authored by Cheng LiCheng Li, Z Liu, C Bath, L Marett, Jennie PryceJennie Pryce, Simone RochfortSimone Rochfort
Short-chain fatty acids (SCFA, C2-C5) in milk and serum are derived from rumen bacterial fermentation and, thus, have the potential to be used as biomarkers for the health status of dairy cows. Currently, there is no comprehensive and validated method that can be used to analyse all SCFAs in both bovine serum and milk. This paper reports an optimised protocol, combining 3-nitrophenylhydrazine (3-NPH) derivatisation and liquid chromatography-mass spectrometry (LC-MS) analysis for quantification of SCFA and β-hydroxybutyric acid (BHBA) in both bovine milk and bovine serum. This method is sensitive (limit of detection (LOD) ≤ 0.1 µmol/L of bovine milk and serum), accurate (recovery 84–115% for most analytes) and reproducible (relative standard deviation (RSD) for repeated analyses below 7% for most measurements) with a short sample preparation step. The application of this method to samples collected from a small cohort of animals allowed us to reveal a large variation in SCFA concentration between serum and milk and across different animals as well as the strong correlation of some SCFAs between milk and serum samples.
Article NumberARTN 436
Rights Statement© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
Science & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemistry & Molecular BiologyChemistry, MultidisciplinaryChemistrybovine milkserumshort-chain fatty acidsliquid chromatography-mass spectrometryQUANTIFICATIONMETABOLISMSTAGEAnimalsCattleChromatography, LiquidFatty Acids, VolatileLimit of DetectionMass SpectrometryMilkReproducibility of ResultsOrganic ChemistryAnalytical Chemistry not elsewhere classifiedAgricultural Biotechnology Diagnostics (incl. Biosensors)