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Omics insights into extracellular vesicles in embryo implantation and their therapeutic utility

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posted on 2023-03-29, 04:47 authored by Qi Hui PohQi Hui Poh, Alin RaiAlin Rai, LA Salamonsen, David GreeningDavid Greening
Implantation success relies on intricate interplay between the developing embryo and the maternal endometrium. Extracellular vesicles (EVs) represent an important player of this intercellular signalling through delivery of functional cargo (proteins and RNAs) that reprogram the target cells protein and RNA landscape. Functionally, the signalling reciprocity of endometrial and embryo EVs regulates the site of implantation, preimplantation embryo development and hatching, antioxidative activity, embryo attachment, trophoblast invasion, arterial remodelling, and immune tolerance. Omics technologies including mass spectrometry have been instrumental in dissecting EV cargo that regulate these processes as well as molecular changes in embryo and endometrium to facilitate implantation. This has also led to discovery of potential cargo in EVs in human uterine fluid (UF) and embryo spent media (ESM) of diagnostic and therapeutic value in implantation success, fertility, and pregnancy outcome. This review discusses the contribution of EVs in functional hallmarks of embryo implantation, and how the integration of various omics technologies is enabling design of EV-based diagnostic and therapeutic platforms in reproductive medicine.

Funding

The work was supported in part by National Health and Medical Research Council project grant (DG: #1139489, 1057741), MRFF Cardiovascular Health Mission (DG: 2015523), Helen Amelia Hains Fellowship (DG) and by the Victorian Government’s Operational Infrastructure Support Program to Research Institutes. QHP is supported by Baker Institute - La Trobe University Research Training Program scholarship.

History

Publication Date

2023-03-01

Journal

Proteomics

Volume

23

Issue

6

Article Number

2200107

Pagination

28p.

Publisher

Wiley

ISSN

1615-9853

Rights Statement

© 2023 The Authors. Proteomics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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