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Molecular Imaging of Activated Platelets Allows the Detection of Pulmonary Embolism with Magnetic Resonance Imaging

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posted on 02.05.2022, 00:11 by Timo Heidt, Simon Ehrismann, Jan-Bernd Hövener, Irene Neudorfer, Ingo Hilgendorf, Marco Reisert, Christoph E Hagemeyer, Andreas Zirlik, Jochen Reinöhl, Christoph Bode, Karlheinz PeterKarlheinz Peter, Dominik von Elverfeldt, Constantin von zur Muhlen

Early and reliable detection of pulmonary embolism (PE) is critical for improving patient morbidity and mortality. The desire for low-threshold screening for pulmonary embolism is contradicted by unfavorable radiation of currently used computed tomography or nuclear techniques, while standard magnetic resonance imaging still struggles to provide sufficient diagnostic sensitivity in the lung. In this study we evaluate a molecular-targeted contrast agent against activated platelets for non-invasive detection of murine pulmonary thromboembolism using magnetic resonance imaging. By intravenous injection of human thrombin, pulmonary thromboembolism were consistently induced as confirmed by immunohistochemistry of the lung. Magnetic resonance imaging after thrombin injection showed local tissue edema in T2∗ weighted images which co-localized with the histological presence of pulmonary thromboembolism. Furthermore, injection of a functionalized contrast agent targeting activated platelets provided sensitive evidence of focal accumulation of activated platelets within the edematous area, which, ex vivo, correlated well with the size of the pulmonary embolism. In summary, we here show delivery and specific binding of a functionalized molecular contrast agent against activated platelets for targeting pulmonary thromboembolism. Going forward, molecular imaging may provide new opportunities to increase sensitivity of magnetic resonance imaging for detection of pulmonary embolism.

Funding

This work was funded by grants of the German Research Foundation (MU2727/6-1) to C.v.z.M., (EL534/6-1) to D.v.E. and institutional funds. J.-B.H. wishes to acknowledge support by the Emmy Noether Programme of the DFG (HO-4604/2-1). K.P. is supported by a principal research fellowship of the National Health and Medical Research Council of Australia. C.E.H. is supported by a fellowship of the National Heart Foundation of Australia.

History

Publication Date

03/05/2016

Journal

Scientific Reports

Volume

6

Issue

1

Article Number

25044

Pagination

9p.

Publisher

Springer Science and Business Media LLC

ISSN

2045-2322

Rights Statement

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/