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Molar concentration from sequential 2-D water-window X-ray ptychography and X-ray fluorescence in hydrated cells

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posted on 2023-02-16, 22:27 authored by MWM Jones, KD Elgass, MD Junker, MD de Jonge, Grant van RiessenGrant van Riessen

Recent developments in biological X-ray microscopy have allowed structural information and elemental distribution to be simultaneously obtained by combining X-ray ptychography and X-ray fluorescence microscopy. Experimentally, these methods can be performed simultaneously; however, the optimal conditions for each measurement may not be compatible. Here, we combine two distinct measurements of ultrastructure and elemental distribution, with each measurement performed under optimised conditions. By combining optimised ptychography and fluorescence information we are able to determine molar concentrations from two-dimensional images, allowing an investigation into the interactions between the environment sensing filopodia in fibroblasts and extracellular calcium. Furthermore, the biological ptychography results we present illustrate a point of maturity where the technique can be applied to solve significant problems in structural biology.

History

Publication Date

2016-04-12

Journal

Scientific Reports

Volume

6

Issue

1

Article Number

24280

Pagination

8p.

Publisher

Springer Nature

ISSN

2045-2322

Rights Statement

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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