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Methyl probes in proteins for determining ligand binding mode in weak protein–ligand complexes

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posted on 2022-10-25, 02:03 authored by B Mohanty, J Orts, Geqing WangGeqing Wang, S Nebl, WS Alwan, BC Doak, ML Williams, Begona HerasBegona Heras, M Mobli, MJ Scanlon
Structures of protein–ligand complexes provide critical information for drug design. Most protein–ligand complex structures are determined using X-ray crystallography, but where crystallography is not able to generate a structure for a complex, NMR is often the best alternative. However, the available tools to enable rapid and robust structure determination of protein–ligand complexes by NMR are currently limited. This leads to situations where projects are either discontinued or pursued without structural data, rendering the task more difficult. We previously reported the NMR Molecular Replacement (NMR2) approach that allows the structure of a protein–ligand complex to be determined without requiring the cumbersome task of protein resonance assignment. Herein, we describe the NMR2 approach to determine the binding pose of a small molecule in a weak protein–ligand complex by collecting sparse protein methyl-to-ligand NOEs from a selectively labeled protein sample and an unlabeled ligand. In the selective labeling scheme all methyl containing residues of the protein are protonated in an otherwise deuterated background. This allows measurement of intermolecular NOEs with greater sensitivity using standard NOESY pulse sequences instead of isotope-filtered NMR experiments. This labelling approach is well suited to the NMR2 approach and extends its utility to include larger protein–ligand complexes.

Funding

Research support for this project was provided by SNF grant 310030_192646 to JO, NHMRC grant 1099151 to MS and BH, and ARC grant IC180100021 to MS. The study was financially supported by the ETH Zurich, the University of Vienna, and Monash University.

History

Publication Date

2022-07-04

Journal

Scientific Reports

Volume

12

Issue

1

Article Number

11231

Pagination

10p.

Publisher

Nature Portfolio

ISSN

2045-2322

Rights Statement

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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