1193182_Tedla,M.G_2022.pdf (2.22 MB)
Download fileMeasuring the Manipulation of T Helper Immune Responses by Schistosoma mansoni
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posted on 2022-07-29, 06:12 authored by MG Tedla, Alison EveryAlison Every, JPY ScheerlinckSchistosoma mansoni uses different mechanisms to escape its host’s immunity. Understanding the ability of memory T cells to withstand this pathogen’s manipulation is important for the development of effective vaccines against this immunomodulatory pathogen. In this study, ovalbu-min (OVA) transgenic S. mansoni is used as a tool to investigate whether fully differentiated Th1, Th2 and Th17 cells are able to withstand pathogen manipulation. Naïve T cells from OT-II T cell receptor transgenic mice with a specificity for OVA were differentiated into Th1, Th2, and Th17 polarised memory cells in vitro. These cells were adoptively transferred into recipient mice to investigate whether these polarised immune memory T cells are resilient in the face of pathogen-mediated manipulation. After transferring memory cells, mice were challenged with OVA-transduced S. mansoni eggs as well as wild-type controls. The in vitro differentiated Th1, Th2 and Th17 memory cells continued to produce the same cytokines when challenged by OVA-expressing S. mansoni eggs as to these they produced when transferred in vivo, suggesting that the Th phenotypes of the memory T cells remains unaltered in the face of stimulation by S. mansoni. The ability of memory T cells to remain resilient to manipulation by the parasite suggests that vaccines might be able to produce immune memory responses able to withstand S. mansoni immune manipulation and hence protect the host from infection.
History
Publication Date
2022-02-01Journal
International Journal of Molecular SciencesVolume
23Issue
3Article Number
ARTN 1462Pagination
12p.Publisher
MDPIISSN
1661-6596Rights Statement
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Publisher DOI
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Keywords
Science & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemistry & Molecular BiologyChemistry, MultidisciplinaryChemistryimmune memoryin vitroin vivoOVAresilienceSmansoniTh memory cellsTH2 POLARIZATIONGENERATIONCELLSPLASTICITYANTIGENPRODUCEPERSISTENCETYPE-1EGGSS. mansoniAdoptive TransferAnimalsAntigensCell PolarityCell ProliferationCytokinesFemaleImmunityImmunologic MemoryLymph NodesMice, Inbred BALB CMice, TransgenicOvalbuminOvumSchistosoma mansoniSchistosomiasis mansoniSpleenT-Lymphocytes, Helper-InducerTh1 CellsTh17 CellsTh2 CellsChemical PhysicsGeneticsBiological Sciences not elsewhere classifiedChemical Sciences not elsewhere classifiedImmunologyParasitologyHost-Parasite Interactions