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Making the most of all data combining non genotyped and genotyped potato individuals with HBLUP.pdf (1.05 MB)

Making the most of all data: combining non-genotyped and genotyped potato individuals with HBLUP

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journal contribution
posted on 24.03.2021, 01:49 by S Sood, Z Lin, Brittney Caruana, AT Slater, Hans Daetwyler
© 2020 The Authors. The Plant Genome published by Wiley Periodicals, Inc. on behalf of Crop Science Society of America Using genomic information to predict phenotypes can improve the accuracy of estimated breeding values and can potentially increase genetic gain over conventional breeding. In this study, we investigated the prediction accuracies achieved by best linear unbiased prediction (BLUP) for nine potato phenotypic traits using three types of relationship matrices pedigree ABLUP, genomic GBLUP, and a hybrid matrix (H) combining pedigree and genomic information (HBLUP). Deep pedigree information was available for >3000 different potato breeding clones evaluated over four years. Genomic relationships were estimated from >180,000 informative SNPs generated using a genotyping-by-sequencing transcriptome (GBS-t) protocol for 168 cultivars, many of which were parents of clones. Two validation scenarios were implemented, namely “Genotyped Cultivars Validation” (a subset of genotyped lines as validation set) and “Non-genotyped 2009 Progenies Validation”. Most of the traits showed moderate to high narrow sense heritabilities (range 0.22–0.72). In the Genotyped Cultivars Validation, HBLUP outperformed ABLUP on prediction accuracies for all traits except early blight, and outperformed GBLUP for most of the traits except tuber shape, tuber eye depth and boil after-cooking darkening. This is evidence that the in-depth relationship within the H matrix could potentially result in better prediction accuracy in comparison to using A or G matrix individually. The prediction accuracies of the Non-genotyped 2009 Progenies Validation were comparable between ABLUP and HBLUP, varying from 0.17–0.70 and 0.18–0.69, respectively. Better prediction accuracy and less bias in prediction using HBLUP is of practical utility to breeders as all breeding material is ranked on the same scale leading to improved selection decisions. In addition, our approach provides an economical alternative to utilize historic breeding data with current genotyped individuals in implementing genomic selection.

Funding

Endeavour Research Fellowship; Agriculture Victoria; LaTrobe University; Hort Innovation

History

Publication Date

01/11/2020

Journal

Plant Genome

Volume

13

Issue

3

Article Number

e20056

Pagination

12p.

Publisher

Wiley

ISSN

1940-3372

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.