Loss of α-actinin-3 during human evolution provides superior cold resilience and muscle heat generation
journal contributionposted on 03.05.2021, 00:04 authored by Victoria Wyckelsma, T Venckunas, PJ Houweling, M Schlittler, VM Lauschke, CF Tiong, HD Wood, N Ivarsson, H Paulauskas, N Eimantas, DC Andersson, KN North, M Brazaitis, H Westerblad
The protein α-actinin-3 expressed in fast-twitch skeletal muscle fiber is absent in 1.5 billion people worldwide due to homozygosity for a nonsense polymorphism in ACTN3 (R577X). The prevalence of the 577X allele increased as modern humans moved to colder climates, suggesting a link between α-actinin-3 deficiency and improved cold tolerance. Here, we show that humans lacking α-actinin-3 (XX) are superior in maintaining core body temperature during cold-water immersion due to changes in skeletal muscle thermogenesis. Muscles of XX individuals displayed a shift toward more slow-twitch isoforms of myosin heavy chain (MyHC) and sarcoplasmic reticulum (SR) proteins, accompanied by altered neuronal muscle activation resulting in increased tone rather than overt shivering. Experiments on Actn3 knockout mice showed no alterations in brown adipose tissue (BAT) properties that could explain the improved cold tolerance in XX individuals. Thus, this study provides a mechanism for the positive selection of the ACTN3 X-allele in cold climates and supports a key thermogenic role of skeletal muscle during cold exposure in humans.
JournalAmerican Journal of Human Genetics
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Muscle, SkeletalAnimalsMice, KnockoutHumansMiceActininCodon, NonsenseBody TemperatureEvolution, MolecularThermogenesisMaleAdipose Tissue, BrownSelection, Geneticalpha-actinin-3 deficincybrown adipose tissueenergy efficient thermogenesisevolutionary advantageimproved cold tolerancemuscle fiber typeskeletal muscleGenetics & Heredity