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Locating and activating molecular 'time bombs': induction of Mycolata prophages

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posted on 2023-03-31, 04:53 authored by Zoe A Dyson, Teagan Brown, Ben Farrar, Stephen R Doyle, Joseph TucciJoseph Tucci, Robert SeviourRobert Seviour, Steve PetrovskiSteve Petrovski
Little is known about the prevalence, functionality and ecological roles of temperate phages for members of the mycolic acid producing bacteria, the Mycolata. While many lytic phages infective for these organisms have been isolated, and assessed for their suitability for use as biological control agents of activated sludge foaming, no studies have investigated how temperate phages might be induced for this purpose. Bioinformatic analysis using the PHAge Search Tool (PHAST) on Mycolata whole genome sequence data in GenBank for members of the genera Gordonia, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella revealed 83% contained putative prophage DNA sequences. Subsequent prophage inductions using mitomycin C were conducted on 17 Mycolata strains. This led to the isolation and genome characterization of three novel Caudovirales temperate phages, namely GAL1, GMA1, and TPA4, induced from Gordonia alkanivorans, Gordonia malaquae, and Tsukamurella paurometabola, respectively. All possessed highly distinctive dsDNA genome sequences.

Funding

ZD was the recipient of an Australian Postgraduate Award PhD Scholarship.

History

Publication Date

2016-08-03

Journal

PLoS One

Volume

11

Issue

8

Article Number

e0159957

Pagination

11p. (p. 1-11)

Publisher

PLOS

ISSN

1932-6203

Rights Statement

© 2016 Dyson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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