La Trobe
1177226_Knox,A_2021.pdf (785.57 kB)
Download file

Isothermal nucleic acid amplification technologies for the detection of equine viral pathogens

Download (785.57 kB)
journal contribution
posted on 2021-08-20, 01:08 authored by Alexandra KnoxAlexandra Knox, Travis BeddoeTravis Beddoe
The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of a viral outbreak causing large epidemics and subsequent significant economic losses. Additionally, horses serve as a host for several zoonotic diseases that could cause significant human health problems. The ability to rapidly diagnose equine viral diseases early could lead to better management, treatment, and biosecurity strategies. Current serological and molecular methods cannot be field-deployable and are not suitable for resource-poor laboratories due to the requirement of expensive equipment and trained personnel. Recently, isothermal nucleic acid amplification technologies, such as loop-mediated isothermal amplification (LAMP) and insulated isothermal polymerase chain reaction (iiPCR), have been developed to be utilized in-field, and provide rapid results within an hour. We will review current isothermal diagnostic techniques available to diagnose equine viruses of biosecurity and zoonotic concern and provide insight into their potential for in-field deployment.


This research was supported by Cooperative Research Centres Project (CRC-P) awarded to Geneworks and La Trobe University. A.K. is supported by an La Trobe Industry PhD scholarship and Defence Science Institute, an initiative of the State Government of Victoria.


Publication Date








Article Number








Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.