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Isothermal nucleic acid amplification technologies for the detection of equine viral pathogens

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journal contribution
posted on 20.08.2021, 01:08 by Alexandra KnoxAlexandra Knox, Travis BeddoeTravis Beddoe
The global equine industry provides significant economic contributions worldwide, producing approximately USD $300 billion annually. However, with the continuous national and international movement and importation of horses, there is an ongoing threat of a viral outbreak causing large epidemics and subsequent significant economic losses. Additionally, horses serve as a host for several zoonotic diseases that could cause significant human health problems. The ability to rapidly diagnose equine viral diseases early could lead to better management, treatment, and biosecurity strategies. Current serological and molecular methods cannot be field-deployable and are not suitable for resource-poor laboratories due to the requirement of expensive equipment and trained personnel. Recently, isothermal nucleic acid amplification technologies, such as loop-mediated isothermal amplification (LAMP) and insulated isothermal polymerase chain reaction (iiPCR), have been developed to be utilized in-field, and provide rapid results within an hour. We will review current isothermal diagnostic techniques available to diagnose equine viruses of biosecurity and zoonotic concern and provide insight into their potential for in-field deployment.

Funding

This research was supported by Cooperative Research Centres Project (CRC-P) awarded to Geneworks and La Trobe University. A.K. is supported by an La Trobe Industry PhD scholarship and Defence Science Institute, an initiative of the State Government of Victoria.

History

Publication Date

20/07/2021

Journal

Animals

Volume

11

Issue

7

Article Number

2150

Pagination

22p.

Publisher

MDPI

ISSN

2076-2615

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