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Investigating the effector suite profile of Australian Fusarium oxysporum isolates from agricultural and natural ecosystems.pdf (961.32 kB)

Investigating the effector suite profile of Australian Fusarium oxysporum isolates from agricultural and natural ecosystems

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journal contribution
posted on 24.03.2021, 01:39 by Saidi Achari, Jatinder K Kaur, Ross C Mann, Tim Sawbridge, Brett A Summerell, Jacqueline Edwards
© 2020 The Authors. Plant Pathology published by John Wiley & Sons Ltd on behalf of British Society for Plant Pathology Pathogenic and putatively nonpathogenic isolates of Fusarium oxysporum are ubiquitously present in soils. Pathogenic isolates designated as formae speciales are very host specific. The genes that determine host-specific pathogenicity may be expected to be similar between strains within a forma specialis. Three different pathways were used for mining putative effectors from 85 assembled genome sequences representing isolates from the agricultural and natural ecosystems. A total of 436 putative effectors were identified, of which 115 were present in all the isolates. The presence–absence phylogeny showed some clustering of isolates based on the ecosystems, while some clustered with isolates from different ecosystems. Isolates from the agricultural and natural ecosystems shared 358 putative effectors. Agricultural isolates had 78 unique putative effectors, while isolates from the natural ecosystems had no unique effectors. Isolates from the natural ecosystems had effectors that have previously been confirmed with virulence functions in other filamentous fungi, suggesting that the natural ecosystems may be acting as a reservoir for pathogenic isolates awaiting compatible hosts. They also possessed putative effectors with domains involved in polysaccharide degradation. The putative effectors in the natural ecosystem isolates may be important for both parasitic and ecological fitness. Further analysis of the three characterized ff. sp.—lycopersici, niveum, and pisi—revealed high conservation in the pathogenicity mechanism in F. oxysporum. However, each strain had unique putative effectors, which may be responsible for host specificity.

Funding

The authors gratefully acknowledge the funding support from the State Government of Victoria through Agriculture Victoria and S.R.A.'s scholarship through La Trobe University, Victoria, Australia. We would like to thank Drs Edward Liew and Matthew Laurence for providing us with 47 isolates from the Royal Botanic Gardens (RBG) Sydney collections, Robyn Brett for the provision of 36 isolates from the Victorian Plant Pathogen Herbarium (VPRI), and the Crop Health Services diagnostics unit for the initial isolations from soil and plant material. The authors would also like to thank Desmond Auer for reviewing the manuscript.

History

Publication Date

01/02/2021

Journal

Plant Pathology

Volume

70

Issue

2

Pagination

10p. (p. 387-396)

Publisher

Wiley

ISSN

0032-0862

Rights Statement

The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.

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