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Interactions between Cellulose and (1,3;1,4)-beta-glucans and Arabinoxylans in Cell Walls of the Ryegrass Lolium multiflorum.pdf (4.75 MB)

Interactions between Cellulose and (1,3;1,4)-β-glucans and Arabinoxylans in the Regenerating Wall of Suspension Culture Cells of the Ryegrass Lolium multiflorum

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journal contribution
posted on 15.03.2021, 02:23 by AVD van de Meene, L McAloney, SM Wilson, JZ Zhou, W Zeng, P McMillan, Tony Bacic, Monika Doblin
Plant cell walls (PCWs) form the outer barrier of cells that give the plant strength and directly interact with the environment and other cells in the plant. PCWs are composed of several polysaccharides, of which cellulose forms the main fibrillar network. Enmeshed between these fibrils of cellulose are non-cellulosic polysaccharides (NCPs), pectins, and proteins. This study investigates the sequence, timing, patterning, and architecture of cell wall polysaccharide regeneration in suspension culture cells (SCC) of the grass species Lolium multiflorum (Lolium). Confocal, superresolution, and electron microscopies were used in combination with cytochemical labeling to investigate polysaccharide deposition in SCC after protoplasting. Cellulose was the first polysaccharide observed, followed shortly thereafter by (1,3;1,4)-β-glucan, which is also known as mixed-linkage glucan (MLG), arabinoxylan (AX), and callose. Cellulose formed fibrils with AX and produced a filamentous-like network, whereas MLG formed punctate patches. Using colocalization analysis, cellulose and AX were shown to interact during early stages of wall generation, but this interaction reduced over time as the wall matured. AX and MLG interactions increased slightly over time, but cellulose and MLG were not seen to interact. Callose initially formed patches that were randomly positioned on the protoplast surface. There was no consistency in size or location over time. The architecture observed via superresolution microscopy showed similarities to the biophysical maps produced using atomic force microscopy and can give insight into the role of polysaccharides in PCWs.

Funding

This research was funded by the Australian Research Council for funding to the ARC Centre of Excellence in Plant Walls (Grant No. CE110100410) and the Thomas Davies Research Grant for Marine, Soil and Plant Biology (awardee A. van de Meene).

History

Publication Date

11/01/2021

Journal

Cells

Volume

10

Issue

1

Article Number

ARTN 127

Pagination

21p.

Publisher

MDPI

ISSN

2073-4409

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