1176192_Hu,Q_2021.pdf (2.05 MB)
In situ monitored vortex fluidic-mediated protein refolding/unfolding using an aggregation-induced emission bioprobe
journal contribution
posted on 2021-08-10, 00:48 authored by Q Hu, H Hu, X Zhang, K Fan, Yuning HongYuning Hong, CL Raston, Y TangProtein folding is important for protein homeostasis/proteostasis in the human body. We have established the ability to manipulate protein unfolding/refolding for β-lactoglobulin using the induced mechanical energy in the thin film microfluidic vortex fluidic device (VFD) with monitoring as such using an aggregation-induced emission luminogen (AIEgen), TPE-MI. When denaturant (guanidine hydrochloride) is present with β-lactoglobulin, the VFD accelerates the denaturation reaction in a controlled way. Conversely, rapid renaturation of the unfolded protein occurs in the VFD in the absence of the denaturant. The novel TPE-MI reacts with exposed cysteine thiol when the protein unfolds, as established with an increase in fluorescence intensity. TPE-MI provides an easy and accurate way to monitor the protein folding, with comparable results established using conventional circular dichroism. The controlled VFD-mediated protein folding coupled with in situ bioprobe AIEgen monitoring is a viable methodology for studying the denaturing of proteins.
Funding
Q. Hu is grateful for the financial support from Postgraduate Research Scholarships (International) for his study at Flinders University. Y. Hong and Y. Tang is grateful for the support from Australia-China Science and Research Fund-Joint Research Centre on Personal Health Technologies. This work is also supported by the Australia Research Council (DP200101106).
History
Publication Date
2021-07-02Journal
MoleculesVolume
26Issue
14Article Number
4273Pagination
(p. 1-11)Publisher
MDPIISSN
1420-3049Rights Statement
The Author reserves all moral rights over the deposited text and must be credited if any re-use occurs. Documents deposited in OPAL are the Open Access versions of outputs published elsewhere. Changes resulting from the publishing process may therefore not be reflected in this document. The final published version may be obtained via the publisher’s DOI. Please note that additional copyright and access restrictions may apply to the published version.Publisher DOI
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