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In Vitro Human Implantation Model Reveals a Role for Endometrial Extracellular Vesicles in Embryo Implantation: Reprogramming the Cellular and Secreted Proteome Landscapes for Bidirectional Fetal‐Maternal Communication
journal contributionposted on 2021-03-15, 05:34 authored by Jemma Evans, Alin Rai, Hong PT Nguyen, Qi Hui PohQi Hui Poh, Kirstin Elglass, Richard SimpsonRichard Simpson, Lois A Salamonsen, David GreeningDavid Greening
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Embryo implantation into maternal endometrium is critical for initiation and establishment of pregnancy, requiring developmental synchrony between endometrium and blastocyst. However, factors regulating human endometrial–embryo cross talk and facilitate implantation remain largely unknown. Extracellular vesicles (EVs) are emerging as important mediators of this process. Here, a trophectoderm spheroid-based in vitro model mimicking the pre-implantation human embryo is used to recapitulate important functional aspects of blastocyst implantation. Functionally, human endometrial EVs, derived from hormonally treated cells synchronous with implantation, are readily internalized by trophectoderm cells, regulating adhesive and invasive capacity of human trophectoderm spheroids. To gain molecular insights into mechanisms underpinning endometrial EV-mediated enhancement of implantation, quantitative proteomics reveal critical alterations in trophectoderm cellular adhesion networks (cell adhesion molecule binding, cell–cell adhesion mediator activity, and cell adherens junctions) and metabolic and gene expression networks, and the soluble secretome from human trophectodermal spheroids. Importantly, transfer of endometrial EV cargo proteins to trophectoderm to mediate changes in trophectoderm function is demonstrated. This is highlighted by correlation among endometrial EVs, the trophectodermal proteome following EV uptake, and EV-mediated trophectodermal cellular proteome, important for implantation. This work provides an understanding into molecular mechanisms of endometrial EV-mediated regulation of human trophectoderm functions—fundamental in understanding human endometrium–embryo signaling during implantation.
J.E., H.N., L.A., and D.G conceived and designed the experiments. J.E., H.N., and D.G. carried out the majority of experiments. J.E. performed the TSC spheroid generation and functional experiments. K.A. performed the confocal microscopy studies. A.R. performed informatics and data interrogation. All authors analyzed the data. J.E., A.R., L.A., and D.G. wrote, reviewed, and edited the manuscript. All authors approved the final manuscript. This work was supported by NHMRC Project grants #1057741 and #1139489, and by the Victorian Government's Operational Infrastructure funding to the Hudson Institute. D.W.G. was supported by La Trobe Institute for Molecular Science Fellowship and La Trobe University Leadership RFA grant. J.E. was supported by a Fielding Fellowship from the Hudson Institute of Medical Research. TSC cells were generously provided by Professor Susan Fisher, University of California, San Francisco, CA, USA.
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Science & TechnologyLife Sciences & BiomedicineBiochemical Research MethodsBiochemistry & Molecular Biologycellular proteomeextracellular vesicleshumanimplantationsecreted proteometrophectodermGENE-EXPRESSIONSTEM-CELLSCROSS-TALKPREIMPLANTATION EMBRYOTROPHOBLAST CELLSMOUSE EMBRYOSESTABLISHMENTPROTEOMEEXOSOMESDIFFERENTIATIONEndometriumEpithelial CellsHumansProteomeCryoelectron MicroscopyMicroscopy, Electron, TransmissionBlotting, WesternCell AdhesionEmbryo ImplantationFemaleEmbryo, MammalianExtracellular Vesicles